许旺细胞在聚羟基乙酸纤维上三维定向培养  

Three-dimensional and oriented culture of Schwann cell on polyglycolic acid fiber

作　　者：戴传昌[1] 曹谊林[2] 王炜[1] 商庆新[2] 董佳生[1] 祁佐良[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科,200011 [2]上海第二医科大学组织工程研究中心

出　　处：《中华显微外科杂志》2000年第4期286-289,共4页Chinese Journal of Microsurgery

摘　　要：目的　为制备组织工程化的神经桥接物。　方法　将体外扩增的许旺细胞 (SCs)接种在纵向排列的、可吸收材料 PGA纤维支架上 ,并对这种立体培养的细胞进行光镜、电镜和免疫组化观察。　结果　发现许旺细胞可与聚羟基乙酸 (PGA)纤维良好吸附 ,并在其上边分裂边迁移从而形成了类似Büngner带的纵向排列的细胞链 ,且其间含有一定的间隙 ,同时细胞大量分泌的层粘蛋白 (L N)沉积在纤维表面形成了纵向的、类似变性神经的结构模式的神经再生引导通道。　结论　这种方法培养 SC具有多种优势 ,因而 。Objective To prepare a novel tissue engineered scaffold to guide axonal regeneration Method Schwann cells (SC) expanded in vitro were inoculated at arranged bioabsorbable polymer polyglycolic acid (PGA) fiber and incubated for two weeks in 5% CO 2 incubator Then the cells were observed in detail under phase contrast microscope and transmission scanning electron microscope,and the creation of laminin from SC was examined by morphology and immunohistochemical study Results The study demonstrated that SCs can adhere to PGA fiber and migrate along fiber,and the SCs polymer formed an ideal three dimensional structure among which SCs ranked into a column along PGA fiber and a tract containing laminin,which was similar to Büngner′s band,and also there was some space,being convenient to substance exchange and survival of the cells,between two adjacent cell columns Conclusion This novel paradigm has many advantages and is hopeful of being applied in research of the peripheral nerve tissue engineering

关 键 词：许旺细胞 细胞培养 聚羟基乙酸 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]