进境意大利苗木中里夫丝剑线虫的鉴定  

Identification of Xiphinema rivesi intercepted from Italy seedlings

作　　者：高菲菲[1] 顾建锋[2] 王江岭[2] 王暄[1] 李红梅[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095 [2]宁波出入境检验检疫局,浙江宁波315012

出　　处：《南京农业大学学报》2013年第4期55-60,共6页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30900939)

摘　　要：2012年春季从宁波口岸进境的意大利地中海柏木和苹果苗木根际介质中检测到1种剑属线虫,通过形态学特征鉴定结合分子序列特征分析的方法,将其鉴定为里夫丝剑线虫(Xiphinema rivesi Dalmasso,1969),是美洲剑线虫组(X.americanum)成员之一。该意大利群体的主要形态鉴别特征为:虫体中等大小,平均长1.9 mm;唇区连续,有时稍缢缩;尾部钝圆锥形,背面弯曲,腹面直或稍弯曲;c'值为1.3～1.9;双生殖管对生,卵巢回折。结合基于rDNA的ITS区和28S的D2/D3区序列构建的系统发育树揭示其与里夫丝剑线虫的亲缘关系最近。里夫丝剑线虫能传播3种植物病毒,是中国进境植物检疫性线虫,目前国内尚无分布的报道,这是中国口岸首次截获。An Xiphinema population was intercepted from the Italy seedlings of Mediterranean cedar(Cupressus sempervirens)and apple(Malus pumila)imported at Ningbo Port in Spring of 2012.Based on the morphological characters and molecular identification,the Italian population was identified as Xiphinema rivesi Dalmasso,1969,which is one of the members of X.americanum group.The morphological characters of the Italian population were mainly as follows:median size body with average length of 1.9 mm;the lip region continuous,sometimes little constricted;the tail bluntly conical,dorsally convex and ventrally straight or slightly convex,c′ value was 1.3-1.9;double gonads opposed and ovary reflexed.The phylogeny trees constructed by sequences of ITS and 28S D2/D3 region of ribosomal DNA revealed that the Italian population is closest to X.rivesi.X.rivesi can transmit 3 kinds of plant viruses and was listed as the imported plant quarantine pest in China.There was no report about the distribution of X.rivesi in China up-to-date and the paper was the first report for the interception from Chinese ports.

关 键 词：里夫丝剑线虫 形态测计 系统发育 意大利 

分 类 号：S41-32[农业科学—植物保护] Q19[生物学—普通生物学]