TAT—XIAP融合蛋白对学习记忆障碍大鼠的影响及其机制  

Effect of TAT-XIAP fusion protein on learning and memory impairment rats and its mechanism

作　　者：夏春波[1] 蒋常文[1] 刘源劼[1] 刘漫君[2] 陈清莲[3] 罗玉琨[3] 蒙丽恒[1] 刘璟[4] 唐金良[2] 覃泱[2] 

机构地区：[1]桂林医学院人体解剖学教研室,桂林541004 [2]桂林医学院附属医院,桂林541004 [3]桂林医学院临床学院,桂林541004 [4]桂林医学院附属第二临床医学院,桂林541004

出　　处：《中华行为医学与脑科学杂志》2013年第7期587-590,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（81260137）;广西高等学校优秀人才资助计划项目（2012-43）;桂林市科学研究与技术开发计划课题（20110119-3）;广西大学生创新创业训练计划资助项目（2012-583;2013-807;2013-808）

摘　　要：目的探讨TAT—XIAP融合蛋白对学习记忆障碍大鼠的影响及其与大脑海马神经元凋亡的关系，为学习记忆的深入研究提供实验依据。方法利用基因工程技术制备具有生物学活性的TAT-XIAP融合蛋白，通过D-半乳糖（D—Gal）腹腔注射和AIM-42海马注射建立学习记忆障碍大鼠模型，将大鼠随机分为TAT—XIAP组（左侧脑室注射TAT—XIAP融合蛋白，每天80mg／kg，共3d）和模型组（侧脑室注射等体积PBS）各30只，采用水迷宫评价大鼠的学习记忆能力，TUNEL法检测大鼠海马神经元凋亡。结果1TAT—XIAP组大鼠在各象限入水至爬上平台的时间均明显缩短，平均值为（48．31±9．44）s，明显低于模型组的（87．80±10．32）S（P〈0．05）；TAT—XIAP组大鼠穿越平台的次数、在原平台象限游程百分比、在原平台象限时间百分比均明显增加，分别为（4．78±0．93）次、（47．39±5．84）％、（48．43±5．75）％，与模型组比较，差异有统计学意义（P〈0．05）。2TAT．XIAP组大鼠海马凋亡神经元明显减少，凋亡神经元计数为（16．37±3．15）个／mm^2，模型组为（54．66±7．20）个／mm^2，两者差异有统计学意义（P〈0．05）。结论TAT—XIAP融合蛋白可明显改善学习记忆障碍大鼠学习记忆功能，其机制可能与大鼠海马神经元凋亡减少有关。Objective To investigate the TAT-XIAP fusion protein on learning and memory impairment in rats and its relationship with the brain hippocampus neuron apoptosis, and to provide experimental evidence for the in-depth study of learning and memory. Methods Using genetic engineering technology to prepare biologically active fusion protein TAT-XIAP, D-galactose （D-Gal） by intraperitoneal injection and Al31-42 by hippocampal in- jection, rat model was established by injection of learning and memory impairment. The rats were randomly divided into TAT-XIAP groups （left lateral cerebral ventricle injection of TAT-XIAP fusion protein, 80 mg/kg per day, 3 days） and model group （lateral cerebral ventricle injection of an equal volume of PBS） ,each group 30 rats. Learning and memory of rats were measured by water maze, and rat hippocampal neuronal apoptosis were measured by TUNEL assay. Results The time was significantly shorter into the water to climb up platform in each quadrant of TAT-XIAP rats, with an average of （48.31 ± 9.44）s, and it was significantly lower than the model group（ （87.80 ± 10.32） s, P 〈 0.05 ）. The number through the platform, target quadrant swim away a percentage and time in the target quadrant percentage were significantly increased in TAT-XIAP group rats （ respectively ,4.78 ± 0.93, （47.39 ± 5.84） %, （48.43 ± 5.75 ） % ）. It was significant difference with the model group comparison （P 〈 0.05 ）. Apoptosis of neuron reduced significantly in hippocampus of TAT-XIAP group rats, apoptosis of neuron count was 16.37 ± 3.15 pieces per square millimeter, and model group was 54.66 ± 7.20 pieces per square millimeter. It was sig- nificant difference compared by statistical analysis（P 〈 0.05 ）. Conclusion TAT-XIAP fusion protein can significantly improve learning and memory function of learning and memory impairment in rats, which may be related to decreasing neurons apoptosis in hippocampus.

关 键 词：学习记忆障碍 X-连锁凋亡抑制蛋白 海马 神经元凋亡 转录反式激活因子 

分 类 号：R285.5[医药卫生—中药学]