大理白族2型糖尿病与脂联素基因启动子SNPs的相关性  

Association of type 2 diabetes with SNPs of adiponectin promoter in the Bai nationality in Dali

作　　者：康庄[1] 赵惠珠 苏恒[1] 张云[1] 虞艳芳[1] 欧杨[1] 薛元明[1] 

机构地区：[1]云南省第一人民医院内分泌科,昆明市650032 [2]大理州人民医院,云南省大理州671000

出　　处：《实用医学杂志》2013年第15期2559-2561,共3页The Journal of Practical Medicine

基　　金：中华医学会临床医学科研专项资金-内分泌疾病研究资金2010年度青年/地区资金资助项目(编号:10020130235);云南省卫生厅内设机构项目(编号:2009NS015)

摘　　要：目的:研究脂联素基因(APM1)启动子区域单核苷酸多态性(single nucleotide polymorphism,SNP)与大理白族2型糖尿病(T2DM)的相关性。方法:运用PCR直接测序技术检测白族正常对照及T2DM患者APM1启动子上的5个SNPs位点:11426A>G、11391G>A、11377C>G、11156insCA、11043C>T的多态性。结果:(1)11391G>A和11043C>T两个位点在白族人群中高度保守;(2)11156insCA位点在正常人群中无碱基插入现象,仅在T2DM患者中存在,但频率非常低;(3)11426A>G和11377C>G位点在两组人群中差异无统计学意义;(4)11426A>G和11377C>G位点相连锁,但在两组人群中差异无统计学意义。结论:APM1启动子上11426A>G、11391G>A、11377C>G、11156insCA、11043C>T这5个多态性位点与大理白族2型糖尿病发生无关。Objective To explore the association of single nucleotide polymorphisms （SNPs） at the proximal promoter region of adiponectin gene （APM1） with type 2 diabetes mellitus （T2DM） in the Bai nationality in Dali. Methods The polymorphisms of APM1 （11426A 〉 G, 11391G 〉 A, 11377C 〉 G, 11156insCA, and 11043C 〉 T） were detected by direct sequencing in nondiabetic controls and patients with T2DM. Results No polymorphisms were found at APMI -11391 and -11043. 11156insCA only existed in T2DM group, with a extremely lower rate of frequency. The frequencies of diversified genotypes and alleles at APM 1 -11426 and -11377 did not differ significantly between the control group and the T2DM group （P 〉 0.05 for all comparisons）. Conclusions 11426A 〉 G, 11391G 〉 A, 11377C 〉 G, 11156insCA, and 11043C 〉 T at the proximal promoter region of APM1 is not associated with the pathogenesis of type 2 diabetes.

关 键 词：糖尿病 2型 脂联素基因启动子 单核苷酸多态性 大理白族 

分 类 号：R587.1[医药卫生—内分泌]