芫菁体内结合斑蝥素对胃癌SGC-7901细胞增殖的抑制作用  

Inhibitory Effect of Bound Cantharidin Extacted from Meloids Mylabris cichorii on the Proliferation of Cell SGC-7901

作　　者：李晓飞[1] 曹嵩[2] 娄方明[3] 晏容[1] 侯晓晖[1] 张恒[1] 刘云[2] 

机构地区：[1]遵义医学院基础医学院 [2]遵义医学院医学与生物学研究中心 [3]遵义医学院药学院,遵义563003

出　　处：《天然产物研究与开发》2013年第7期963-966,共4页Natural Product Research and Development

基　　金：国家自然科学基金项目(81260488);贵州省优秀科技教育人才省长资金(2010-48);贵州省中药现代化科技产业研究开发专项项目(2009-5042);贵州省科技厅社会发展攻关项目(2011-3031)

摘　　要：本文考察了芫菁体内结合斑蝥素和人工合成的斑蝥素盐类衍生物斑蝥素酸镁的体外抗肿瘤活性。采用WST-1法检测两者在体外对人胃腺癌SGC-7901细胞增殖的抑制作用。实验结果显示两者对SGC-7901细胞均表现出明显的抑制效果,且随药物浓度升高其抑制作用增强,呈剂量效应关系;其半数抑制浓度(IC50)分别为10.86和8.65μmol/L。此外,通过流式细胞术检测表明,结合斑蝥素能引起SGC-7901细胞G0～G1期阻滞;斑蝥素酸镁则引起SGC-7901细胞S期阻滞,两者均能通过干预SGC-7901细胞的周期来抑制其增殖。The study inversitigated the anti-tumor activity of bound cantharidin extacted from meloids Mylabris cichorii and magnesium cantharidate obtained by chemosynthesis in vitro. WST-1 colorimetry was used to evaluate the inhibitory effect of bound cantharidin and magnesium cantharidate on human gastric cancer cell line SGC-7901. The results showed they displayed obvious inhibitory effect on the growth of SGC-7901 cells in a dose-dependent manner, and the IC50 value were 10.86 and 8.65 μmol/L,respectively. Moreover,the results of flow cytometer showed that the G0-G1 phase was ar- rested by bound cantharidin,while S phase was arrested by magnesium cantharidate. They could inhibit proliferation on SGC-7901 cells by cell cycle disturbing.

关 键 词：芫菁 结合斑蝥素 斑蝥素酸镁 人胃腺癌SGC-7901细胞 细胞增殖 

分 类 号：R284.2[医药卫生—中药学]