毛茛有效部位D1对人胃癌细胞MGC803细胞增殖和凋亡的作用及其机制的初步研究  

Preliminary Study of Effect and Mechanisms of Ranunculus japonicus Component D1 on Human Gastric Cancer Cell MGC803 Proliferation and Apoptosis

作　　者：朱峰妍[1] 虞迪[2] 成旭东[1] 曹志飞[2] 王薇丹[1] 鲍美美[2] 蒋小岗[1] 顾振纶[1] 

机构地区：[1]苏州大学苏州中药研究所,苏州215007 [2]苏州大学医学部,苏州215123

出　　处：《天然产物研究与开发》2013年第7期967-971,948,共6页Natural Product Research and Development

基　　金：苏州市科技支撑计划项目(SS201004)

摘　　要：采用AlamarBlue和瑞-姬氏染色方法检测毛茛有效部位D1对人胃癌细胞MGC803增殖的作用;PI染色法观察D1对MGC803细胞周期的影响;半定量RT-PCR法检测D1对MGC803细胞周期相关基因表达的作用;用DAPI染色方法和细胞色素C免疫荧光法观察D1对胃癌细胞MGC803凋亡的影响。结果显示,毛茛有效部位D1对胃癌细胞MGC803表现出较好的增殖抑制作用,且24、48和72 h的半数抑制浓度分别为126.89、74.81、68.72μg/mL;同时D1对细胞周期和周期相关基因的表达无明显影响,且D1能促使细胞色素C释放到细胞胞浆诱导细胞凋亡。Alamar Blue assay and Wright-Giemsa assay were used to determine the effect of Rannunculus japonicas com- ponent D1 on the proliferation of human gastric cancer cell MGC803. The effect of D1 on the cell cycle distribution of MGC803 was observed by PI assay and semi-quantitative RT-PCR assay was used to detect the expression of cell cycle related genes. The effect of D1 on cell apoptosis of MGC803 was observed by DAPI assay and Cytoehrome C immunofluo- rescence assay. The results showed that D1 obviously inhibited MGC803 proliferation and the ICs0 values of 24,48 and 72 h were about 126.89,74.81 and 68.72 μg/mL,respectively. However,D1 did not obviously induce MGC803 cell cy- cle arrest and did not markedly affect the expression of cell cycle related genes. Furthermore, D1 induced cell apoptosis by promoting cytochrome C releasing tO cytoplasm.

关 键 词：毛茛 人胃癌细胞 MGC803 细胞色素C 细胞凋亡 

分 类 号：R284[医药卫生—中药学]