国产齐考诺肽注射液对大鼠镇痛效果的影响  

Analgesic Effects of Domestic Ziconotide Injection in Rats

作　　者：屠伟峰[1] 邓丽珍[1] 马亚平 尹晴[1] 杨学敏 康旭 聂涛 

机构地区：[1]广州军区广州总医院麻醉科/全军临床麻醉中心,广州510010 [2]深圳翰宇药业股份有限公司/多肽药物国家地方联合工程实验室,广东深圳518057

出　　处：《中国药房》2013年第29期2710-2712,共3页China Pharmacy

基　　金：广东省科技计划资助项目(No.2012A080202012)

摘　　要：目的:研究国产齐考诺肽注射液对大鼠腰5脊神经病理性疼痛的抑制作用。方法:将大鼠随机分为模型组,国产齐考诺肽低、中、高剂量(每只30、100、300ng)组,进口齐考诺肽低、中、高剂量(每只30、100、300ng)组,每组8只。用神经结扎加切断术建立腰5脊神经病理性疼痛模型,术后第7天鞘内注射相应药物,检测给药后1、2、4、8h的50%机械性撤足阈值(50%PWT),计算最大效应百分比(MPE)。结果:与模型组比较,国产和进口齐考诺肽低、中、高剂量组大鼠给药后各时间点的50%PWT、MPE均明显增加(P<0.01),且呈明显量效关系;与同剂量的进口齐考诺肽组比较,国产齐考诺肽中、高剂量组大鼠给药后1、2、4h的50%PWT、MPE差异无统计学意义(P>0.05)。结论:国产和进口齐考诺肽注射液对大鼠腰5脊神经病理性疼痛均具有较好的抑制作用。OBJECTIVE：To study the inhibitory effect of domestic Ziconotide injection on neuropathic pain induced by L5 spinal nerve ligation（L5 SNL） in rats.METHODS：Rats were randomly divided into model group,domestic Ziconotide injection low-dose,medium-dose and high-dose groups（30,100,300 ng/rat）,imported Ziconotide injection low-dose,medium-dose and high-dose groups（30,100,300 ng/rat）with 8 rats in each group.Meuropathic pain model was induced by L5 SNL plus neurectomy in rats.They were given relevant medicine intrathecally 7 d after operation.The 50% paw withdrawal thresholds（50%PWT） were measured 1,2,4,8 h after medication,and the maximum possible effect（MPE）was calculated.RESULTS：Compared with model group,50% PWT and MPE were significantly increased in domestic and imported Ziconotide injection low-dose,medium-dose and high-dose groups（P0.01）,showing a significant dose-dependent manner;compared with imported Ziconotide injection group at the same dose,50%PWT and MPE of domestic Ziconotide injection medium-dose and high-dose groups were no significant differences 1,2,4 h after medicayion（P0.05）.CONCLUSIONS：Domestic and imported Ziconotide injection have a sound analgetic effect on the neuropathic pain induced by L5 SNL in rats.

关 键 词：齐考诺肽 进口 国产 大鼠 神经病理性疼痛 50%机械性撤足阈值 最大效应百分比 

分 类 号：R965[医药卫生—药理学]