金福安汤对小鼠Lewis肺癌生长的抑制作用及其分子机制的研究  

Inhibitory Effect of Jinfuan Decoction on Mice Lewis Lung Cancer and Its Molecular Mechanism

作　　者：曹洋[1] 林洁衡[1] 任明能[1] 谢宇晖[1] 吴玉生[1] 李丹青[1] 周健洪[2] 陈文华[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学基础医学院,广东广州510006

出　　处：《中药新药与临床药理》2013年第4期329-333,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(30901903)

摘　　要：目的观察金福安汤对小鼠Lewis肺癌生长的抑制作用及其相关的分子机制。方法将50只Lewis肺癌荷瘤小鼠分为金福安汤高、中、低剂量组,环磷酰胺(CTX)组,模型组;给药结束后计算各组小鼠平均瘤体质量、抑瘤率、肺表面转移灶及抗转移率;采用免疫组化法检测各组Lewis肺癌小鼠的p120ctn表达以及RhoA、Cdc42表达。结果与模型组比较,金福安汤中剂量组及CTX组的瘤体质量明显减轻(P<0.05);金福安汤中、高剂量组及CTX组小鼠的肺转移灶数明显减少(P<0.01,P<0.05);金福安汤中剂量组及CTX组的p120ctn异常表达细胞数明显减少(P<0.01,P<0.05);金福安汤中剂量组和CTX组的RhoA、Cdc42的异常表达细胞数均明显减少(P<0.01)。结论金福安汤具有抑制小鼠Lewis肺癌生长及转移的作用,其机理可能与抑制p120ctn的异常表达,进而抑制RhoA和Cdc42的异常表达相关。Objective To observe the inhibitory effect of Jinfuan decoction on Lewis lung cancer in mice and to explore its molecular mechanism.Methods Fifty mice transplanted with Lewis lung cancer were randomly divided into model control group,Cytoxan（CTX）group,and high-dose,middle-dose and low-dose Jinfuan Decoction groups.The average tumor mass weight,tumor-inhibitory rate,pulmonary metastasis foci,and anti-metastasis rate were calculated in each group.The expression levels of p120ctn,RhoA and Cdc42 were examined by immunohistochemical method.Results The tumor mass weight was statistically lighter in CTX group and middle-dose Jinfuan Decoction group than that in the model control group（P0.05）.The number of pulmonary metastasis foci and cells with abnormal expression of p120ctn were statistically less in CTX group and middle-dose Jinfuan Decoction groups than those in the model control group（P0.01 or P0.05）.And the abnormal expression RhoA and Cdc42 was also statistically de creased in middle-dose Jinfuan Decoction group（P0.01）.Conclusion Jinfuan Decoction has inhibitory effect on the growth and metastasis of Lewis lung cancer in mice,and its therapeutic mechanism is probably related with the inhibition of abnormal expression of p120ctn,RhoA and Cdc42 in mice.

关 键 词：金福安汤 肺癌 P120CTN RHOA CDC42 

分 类 号：R285.5[医药卫生—中药学]