罗氏内异方含药血清对子宫内膜异位症合并不孕患者腹腔液细胞的影响  

Influence of Serum Containing Luoshi Neiyi Recipe on Peritoneal Fluid Cells in Endometriosis Complicated with Infertility Patients

作　　者：史云[1] 伍海鹰 何金洋[3] 陶莉莉[1] 

机构地区：[1]广州中医药大学第一附属医院妇科,广东广州510405 [2]广州市妇女儿童医疗中心妇科,广东广州510400 [3]广州中医药大学热带医学研究所,广东广州510405

出　　处：《中药新药与临床药理》2013年第4期344-347,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(83018)

摘　　要：目的探讨罗氏内异方对子宫内膜异位症合并不孕患者腹腔液细胞的影响。方法分离、培养子宫内膜异位症不孕患者腹腔液细胞,采用罗氏内异方与内美通含药血清刺激6 h,荧光定量聚合酶链反应测定血管内皮生长因子(VEGF)、肿瘤坏死因子α(TNF-α)、血管内皮生长因子受体1(FLT-1)与血管内皮生长因子受体4(FLT-4)mRNA表达水平的变化。结果与生理盐水组比较,罗氏内异方高剂量组TNF-αmRNA显著降低(P<0.01),高、中剂量组VEGF mRNA均显著降低(P<0.01),高、中、低剂量组FLT-1 mRNA均显著降低(P<0.05);高、中剂量组FLT-4 mRNA均显著降低(P<0.01)。结论罗氏内异方治疗轻型子宫内膜异位症不孕症的机制可能是通过降低患者腹腔液细胞VEGF、TNF-α、FLT-1、FLT-4 mRNA的表达,改善腹腔内环境而实现的。Objective To study the influence of Luoshi Neiyi Recipe on peritoneal fluid cells in endometriosis complicated with infertility patients.Methods Peritoneal fluid cells were isolated from endometriosis complicated with infertility patients,and then were cultured in vitro with serum containing different concentrations of Luoshi Neiyi Recipe and Nemestran for 6 hours.Vascular endothelial growth factor（VEGF）,tumor necrosis factor alpha（TNF-α）,growth factor receptors 1 and 4（FLT-1 and FLT-4）mRNA expression levels were detected by fluorescence quantitative polymerase chain reaction（PCR）.Results Compared with the normal saline control group,TNF-α mRNA expression in high-dosage Luoshi Neiyi Recipe group was reduced obviously（P 0.01）,VEGF and FLT-4 mRNA was reduced in high-and medium-dosage Luoshi Neiyi Recipe groups（P 0.01）,and FLT-1 mRNA was reduced in high-,medium-and low-dosage of Luoshi Neiyi Recipe groups（P 0.05）.Conclusion The mechanism of Luoshi Neiyi Recipe in the treatment of mild endometriosis complicated with infertility is probably related with the reduction of VEGF,TNF-α,FLT-1 and FLT-4 mRNA expression in peritoneal fluid cells and with the regulation of abdominal internal environment.

关 键 词：罗氏内异方 子宫内膜异位症 不孕症 腹腔液细胞 

分 类 号：R285.5[医药卫生—中药学]