麻黄连轺赤小豆汤三方对H_2O_2诱导损伤的L-O2人肝细胞的保护作用  

Protective Effect of Three Kinds of Mahuang Lianqian Chixiaodou Decoction on L -O2 Hepatocyte Injury Induced by H_2O_2

作　　者：吴军[1] 周春祥[1] 赵凤鸣[1] 周韬[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046

出　　处：《中药新药与临床药理》2013年第4期367-370,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省研究生科研创新计划项目(2010)

摘　　要：目的研究麻黄连轺赤小豆汤三种组方(简称"三方")对体外肝细胞损伤的保护作用及其机制。方法培养L-O2人肝细胞,采用H2O(2100μmol.L-1)体外诱导肝细胞损伤,检测细胞上清中天门冬氨酸转换酶(AST)和丙氨酸氨基转换酶(ALT)、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活力,cck-8法检测细胞活性。结果与阴性对照组比较,H2O(2100μmol.L-1)可抑制肝细胞活性,增加MDA的生成和ALT、AST的水平,降低SOD的活力(P<0.01,P<0.05);与模型组比较,麻黄连轺赤小豆汤三方均可拮抗H2O2对肝细胞的损伤作用,可促进细胞增殖,显著降低细胞上清ALT(除楸树皮方低剂量组)和AST水平(除楸树皮方高剂量组)及MDA含量(除梓白皮方低剂量组),明显提升SOD活力(P<0.01,P<0.05)。结论麻黄连轺赤小豆汤三方对体外肝细胞损伤均有直接保护作用,该作用可能与其抗氧化作用有关。Objective To observe the protective effect of Mahuang Lianqian Chixiaodou Decoction（MLCD）,including the original recipe,and two kinds of variation recipes with Sangbaipi（Cortex Mori）,Qiushupi（Cortex Catalpae Gungei）taking the place of Zibaipi（Cortx Catalpae Ovatae Radicis）,on L-O2 hepatocyte injury induced by H2O2 and to reveal the mechanism.Methods L-O2 hepatocytes were incubated,and then injured by H2O2.The levels of aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,and malondialdehyde（MDA）,and superoxide dismutase（SOD）activity in the supernatant were detected.Cell proliferation was assayed by cck-8 reduction method.Results Compared with negative control group,H2O2 at 100 μmol.L-1 inhibited the activity of L-O2 hepatocytes,increased MDA,ALT and AST levels,and decreased SOD activities.And Mahuang Lianqian Chixiaodou Decoction counteracted the above changes（P0.05 or P0.01）,except for ALT in middle-dose Qiushupi-MLCD,AST in high-dose Qiushupi-MLCD,and MDA in middle-dose Zibaipi-MLCD.Conclusion Mahuang Lianqian Chixiaodou Decoction has protective effect on H2O2-induced hepatocytes injury in vitro,and the mechanism might be associated with its anti-oxidation activity.

关 键 词：麻黄连轺赤小豆汤 L-O2肝细胞 过氧化氢 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]