三七总皂苷对3T3-L1细胞增殖分化与分泌功能的影响  

Effect of Panax Notoginsenosides on Proliferation,Differentiation and Secretion of 3T3-L1 Preadipocytes

作　　者：刘新迎 周联[2] 王培训[2] 

机构地区：[1]佛山市南海区罗村医院,广东佛山528226 [2]广州中医药大学,广东广州510405

出　　处：《中药新药与临床药理》2013年第4期379-382,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的观察三七总皂苷对3T3-L1细胞增殖、分化和分泌Leptin、PAI-1的影响,探讨其调脂作用的可能机制。方法培养3T3-L1细胞,并用不同浓度的三七总皂苷(5,50,100μg.mL-1)进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖;用油红O染色和染色比色法分析脂肪细胞的分化程度,用逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ(2PPARγ)2、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达;用ELISA法检测细胞培养上清中Leptin、PAI-1的含量。结果三七总皂苷低、中浓度组对模型细胞增殖无明显影响(P>0.05),高浓度组能促进细胞的增殖(P<0.05);中、高浓度组明显抑制模型细胞的分化,并能抑制PPARγ2、C/EBPα基因的表达(P<0.01);药物对模型细胞分泌Leptin、PAI-1有明显抑制作用(P<0.01)。结论三七总皂苷可能通过抑制3T3-L1前脂肪细胞的分化和抑制其分泌因子而达到调脂作用,以中剂量作用效果最好。Objective To investigate the effect of Panax Notoginsenosides（PNS）on the proliferation and differentiation of 3T3-L1 preadipocytes and their secretion of Leptin and PAI-1,and to explore the possible lipid-regulating mechanism.Methods Cultured 3T3-L1 cells were incubated with PNS at the concentrations of 5,50,100 μg.mL-1,and then MTT method was used to detect the cell proliferation,oil red O staining method and spectrophotography were used to analyze the degree of cell differentiation.The expression of adipocytes differentiation-associated genes of peroxisome proliferator-activated receptor γ（2PPARγ2）and CCAAT/enhancer binding protein α（C/EBPα）mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）.ELISA technique was used to detect Leptin and PAI-1 contents in the culture supernatant of mature fat cells.Results Low-and middle-dose PNS had no effect on the proliferation of 3T3-L1,but high-dose PNS promoted the proliferation of 3T3-L1.Middle-and high-dose PNS inhibited the differentiation of 3T3-L1 and down-regulated the expression of PPARγ2 and C/EBPα mRNA.PNS obviously inhibited the secretion of Leptin and PAI-1 from the model cells.Conclusion PNS can regulate the lipid metabolism through inhibiting the differentiation of preadipocytes and inhibiting the secretion function of the cells,and middle dose has the best action.

关 键 词：三七总皂苷 3T3-L1细胞 增殖分化 分泌功能 

分 类 号：R285.5[医药卫生—中药学]