淫羊藿对骨质疏松MSCs成脂分化PPARγ mRNA表达的影响  

Effect of Herb Epimedii on Expression of PPARγ mRNA of MSCs During Adipogenesis in postmenopausal osteoporosis rats

作　　者：刘海全[1] 秦佳佳[2] 吴倩[1] 

机构地区：[1]广州中医药大学附属骨伤科医院,广东广州510240 [2]暨南大学医学院,广东广州510632

出　　处：《中药新药与临床药理》2013年第4期382-385,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30901915);广东省普通高校“中医女性生殖调节与安全性研究”重点实验室开放基金资助项目(ZA201112);广东省自然科学基金资助项目(S2011010004277)

摘　　要：目的探讨淫羊藿含药血清在骨质疏松大鼠骨髓间充质干细胞(MSCs)成脂分化过程中对过氧化物酶体增殖物激活受体亚型PPARγmRNA表达的的影响。方法分离、培养骨质疏松大鼠MSCs,传至3代。将所获细胞随机分为模型组,成脂诱导组,西药组,成脂诱导西药组,中药组,成脂诱导中药高、中、低剂量组。采用淫羊藿含药血清对MSCs进行干预,并与尼尔雌醇含药血清进行对照,观察各组PPARγmRNA表达的变化。结果与模型组比较,西药组、中药组PPARγmRNA表达明显降低(P<0.01,P<0.05);与成脂诱导组比较,成脂诱导西药组、成脂诱导中药高、中、低剂量组PPARγmRNA表达明显降低(P<0.05,P<0.01)。结论淫羊藿含药血清可抑制绝经后骨质疏松症大鼠MSCs及其在成脂分化过程中PPARγmRNA的表达,从而抑制MSCs成脂分化,以防治骨质疏松症。Objective To observe the effect of Herb Epimedii on PPARγ mRNA expression of MSCs during adipogenesis in postmenopausal osteoporosis rats.Methods The third generation of MSCs were divided into 8 groups at random,model group（MG）,adipogenic induction group（AIG）,western medicine group（WMG）,adipogenic western medicine group（AWMG）,herb group（HG）,adipogenic low-dose herb group（ALDHG）,adipogenic medium-dose herb group（AMDHG）,adipogenic high-dose herb group（AHDHG）.The PPARγ mRNA expression of each group was observed.Results Compared with the MG,PPARγ mRNA expression of WMG and HG were decreased（P 0.05,P0.01）.Compared with the AIG,PPARγ mRNA expression of AWMG,AWMG,ALDHG and AMDHG were decreased（P 0.05,P 0.01）.Conclusion Herb Epimedii can reduce PPARγ mRNA expression of MSCs in postmenopausal osteoporosis rats,inhibit the adipogenesis of MSCs,which contributes to the mechanism of the role of prevention and treatment of osteoporosis.

关 键 词：淫羊藿 骨髓间充质干细胞 成脂分化 PPARΓ 

分 类 号：R285.5[医药卫生—中药学]