高效液相色谱法测定龙眼果实中ATP、ADP、AMP的含量  

Determination of the Content of ATP, ADP, AMP in Longan Fruit Using High Performance Liquid Chromatography

作　　者：杨子琴[1,2] 王惠聪[1] 付欣雨[1] 李建国[1] 黄旭明[1] 

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《热带作物学报》2013年第7期1381-1383,共3页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30871693;31171919);广东省高等学校高层次人才项目;中央级公益性科研院所基本科研业务费专项资金(中国热带农业科学院热带作物品种资源研究所)资助项目(No.1630032012016)

摘　　要：摸索了一种基于高效液相色谱仪快速测定龙眼果实腺苷三磷酸（ATP）、腺苷二磷酸（ADP）、腺苷一磷酸（AMP）含量的方法。该方法利用磷酸盐缓冲液（pH7.0）作为单一流动相,流速设定为1 mL/min,停止时间13 min,检测波长254 nm,无参比波长。ATP、ADP和AMP浓度在5~50 mg/L范围内与相应的色谱峰面积呈完美的直线关系（决定系数R2均为1.000）,回收率95.2%~97.5%,保留时间分别为8.651、9.844和11.457 min。该方法测定结果表明,果实腺苷含量水平分别为30.0、6.5和2.0μg/g,能荷值为0.86。A rapid method using high performance liquid chromatography was explored to determine the levels of adenosine triphosphate,adenosine diphosphate,adenosine monophosphate in longan fruit.The method used 0.1 mol/L KH2PO4（pH7.0） as the single mobile phase with a flow rate of 1 mL/min,stop time 13 min,the detection wavelength 254 nm,without reference wavelength.The retention time for ATP,ADP and AMP was 8.651,9.844 and 11.457 min,respectively.Within 0~50 mg/L,ATP,ADP and AMP concentrations all displayed perfect linear correlation（R2= 1.000） with their peak areas.The recovery rate reached 95.2% ~97.5%.This method was applied to determine levels of adenosine phosphates in young longan fruit,and the results showed that the average contents of ATP,ADP and AMP were 30.0,6.5 and 2.0 μg/g,respectively,with an energy charge of 0.86 in young fruit.

关 键 词：高效液相色谱 龙眼 腺苷三磷酸 腺苷二磷酸 腺苷一磷酸 

分 类 号：S667.2[农业科学—果树学]