羊口疮病毒感染对巨噬细胞RAW264.7凋亡的影响  

The Influence on Apoptosis of Macrophage RAW264.7 during ORFV Infection

作　　者：于永忠[1] 柳婧[1] 孙虎男[1] 冯丽[1] 崔玉东[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《中国畜牧兽医》2013年第7期25-28,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金面上项目(31172353);黑龙江省教育厅科学技术研究项目(11551322)

摘　　要：羊口疮(Orf)是由羊口疮病毒(ORFV)引起的一种急性、接触性和高度嗜上皮性人畜共患的动物传染病,主要感染绵羊和山羊,有时威胁人类。本研究将ORFV(OV/HLJ/04株)纯化后作为试验材料,采用巨噬细胞系RAW264.7作为病毒细胞宿主,RAW264.7在LPS体外激活的情况下,接种ORFV,研究ORFV感染对巨噬细胞凋亡的影响。结果表明,用LPS处理的试验组巨噬细胞形态学改变较大,凋亡量也较大;而没用LPS处理过的试验组巨噬细胞变化则相对较少。Westernblotting研究结果显示,细胞凋亡信号Caspase-3与甘油醛-3-磷酸脱氢酶(GAPDH)在24h时对比,凋亡信号明显减弱,表明在24h的巨噬细胞凋亡水平有所下降。Orf was an acute skin zoonosis caused by Orf virus （ORFV）, which could affect sheep, goats and humans. This research used purified ORFV （OV/HLJ/4 strains） as experimental material, the macrophage cell line RAW264. 7 as host, RAW264.7 were classically activated by LPS before ORFV inoculation in vitro, the effect for macrophage apoptosis upon OR- FV infection was investigated simultaneously. Comparison of morphological features of activation in the ceils treated with I.PS and non-LPS, these resulted in significant morphological variation each other. The apoptosis quantity of macrophage cells trea- ted with the LPS was bigger than the non-LPS group which was less of relative changes. Western blotting showed that the ap- optosis signal Caspase-3 weakened at 24 h comparing with glyceraldehyde 3-phosphate dehydrogenase （GAPDH）, such a pop tosis signal that was significantly weakened, related to that macrophage apoptosis level declined at that time.

关 键 词：羊口疮病毒 巨噬细胞 细胞凋亡 差异性 

分 类 号：S482[农业科学—农药学]