正交试验法优化三芪丹颗粒醇提工艺  

Optimization of Extraction Process of Sanqidan Granules by Orthogonal Test

作　　者：李丽娟[1] 李素梅[2] 罗文汇[2] 范宋玲[1] 

机构地区：[1]广东省第二中医院,广东广州510095 [2]广东省中医研究所,广东广州510095

出　　处：《亚太传统医药》2013年第7期22-24,共3页Asia-Pacific Traditional Medicine

基　　金：广东省中医药强省项目(20111128)

摘　　要：目的:优化三芪丹颗粒的醇提工艺。方法:采用正交试验对三七的醇提工艺进行优化,以三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1提取量及出膏率作为考察指标,以乙醇浓度、乙醇用量、提取次数、提取时间作为考察因素,用L9(34)正交试验表安排试验。结果:最佳提取工艺为加6倍量60%乙醇提取3次,每次1.5h。结论:优选工艺科学合理,可作为该制剂合理开发的依据。Objective：To establish the optimal extraction process of Sanqidan granules. Methods： Orthogonal test was employed for selecting the optimum ethanol extraction process of Notoginseng the indexes of the contents of notoginsenoside R1 , ginsenoside Rg1, ginsenoside Rbl, and total solid matters. Study factors were ethanol concentration, ethanol-adding amount, extraction times, extracting time. The L9 （3＋4） Orthogonal table was used to arrange experiment. Results：The best extraction condition was as follows： 6 times of 60 % alcohol, extracted for 3 times, each time for 1.5 hours. Conclusion：The optimum extraction process is rea sonable and stable and it can provide the references forSanqidan granules for its further investigation.

关 键 词：三芪丹颗粒 正交试验 三七皂苷 人参皂苷 

分 类 号：R284.2[医药卫生—中药学]