云南白药水提取物抑制铜绿假单胞菌AlgR2-AlgR1双组份调控系统磷酸化研究  

Study of the Inhibitory Effect of Aqueous Extract of Yunnan Baiyao on the Phosphorylation of AlgR2-AlgR1 Two-Component Regulatory System of Pseudomonas aeruginosa

作　　者：赵祖国[1] 赵静 刘军[1] 张腊喜 喻云梅 

机构地区：[1]广东医学院微生物与免疫学教研室,广东湛江524023 [2]广东省解放军第422医院,广东湛江524005

出　　处：《亚太传统医药》2013年第7期31-33,共3页Asia-Pacific Traditional Medicine

基　　金：广东医学院博士启动项目(B2010005);湛江科技计划项目(2012C3101045)

摘　　要：目的:研究亚致死剂量的云南白药水提取物(Aquatic Extract of Yunnan Baiyao,AEYB)对铜绿假单胞菌(Pseudomonas Aeruginosa,PA)AlgR1-AlgR2双组份调控系统的磷酸化、海藻酸盐的生物合成及生物膜形成的影响。方法:通过放射性同位素标记方法研究AEYB对AlgR1-AlgR2磷酸化的影响;采用real-time PCR分析YBX海藻酸盐合成酶基因algD转录的影响;用咔唑法和结晶紫法分别对海藻酸盐和静态生物膜进行定量测定。结果:AEYB并不抑制AlgR2的自磷酸化,但能抑制AlgR2的磷酸基团向AlgR1转移,从而抑制AlgR1的磷酸化。AEYB可显著下调algD基因的转录,并抑制alginate的合成,最终减少PA静态生物膜的形成。结论:云南白药可通过抑制AlgR1-AlgR2系统的磷酸化的途径来减少PA的alginate的生物合成,对PA生物膜的形成具有抑制作用。Objective：Study on the Inhibitory Effect of sublethal doses of Yunnan Baiyao aqueous extract on the phosphorylation of AlgR1-AlgR2 two-component regulatory system, and alginate biological synthesis and biofilm formation ofPseudomonas aeruginosa. Methods：Phosphorylation of Algrl and Algr2 was detected by radioisotope method, transcription ofalgD was quantified by real- time PCR, and synthesis of alginate was determined by carbazole analysis, and 24 h static biofilm was quantified with crystal violet. Results：AEYB didn＇t affect the self-phosphorylation of AlgR2, but can inhibit the transfer of phosphate group from AlgR2 to AlgR1, thus inhibiting the phosphorylation of AlgR1, which lead to significant down-regulation of the transcriptional level ofalgDgene, and the synthesis of alginate and biomass of PA biofilm. Conclusion： YB can reduce the synthesis of alginate and biofilm formation by inhibiting the phosphorylation of AlgR-AlgR2 two-component regulatory system.

关 键 词：云南白药 铜绿假单胞菌 海藻酸盐 AlgR 生物膜 

分 类 号：R285.5[医药卫生—中药学]