nHA-PLGA支架材料与兔软骨细胞体外培养的生物相容性研究  

Biocompatibility of nHA/PLGA composite scaffolds with rabbit chondrocytes cultured in vitro

作　　者：陈磊[1,2] 王大平[2] 朱伟民[2] 费志强[2] 陈洁琳[2] 雷鸣[2] 熊建义[2] 李映红[2] 黄江鸿[2] 

机构地区：[1]广州医科大学,510182 [2]深圳市第二人民医院骨科深圳市组织工程重点实验室,518035

出　　处：《国际骨科学杂志》2013年第4期283-286,共4页International Journal of Orthopaedics

基　　金：深港创新圈资助计划(JSE201007190007A)

摘　　要：目的研究新型多孔复合支架材料纳米羟基磷灰石(nHA)-聚乳酸羟基乙酸共聚物(PLGA)的生物相容性,探讨其作为骨组织工程支架的可行性。方法将胎兔膝关节软骨细胞接种于制备的nHA-PLGA复合支架上,体外共同培养后采用四甲基偶氮唑蓝(MTT)法检测软骨细胞增殖活性,倒置荧光显微镜、扫描电镜观察支架材料表面和孔隙内软骨细胞黏附情况,流式细胞术检测软骨细胞周期情况。结果实验组(A组)复合支架材料上细胞增殖活性与空白对照组(B组)相比,无统计学差异(P>0.05);倒置荧光镜、扫描电镜观察显示A组细胞在复合支架材料表面和孔隙内大量黏附、生长,其数量随着共培养时间增加呈几何级增长;流式细胞仪检测显示A组与B组细胞周期差异无统计学意义(P>0.05)。结论 nHA-PLGA多孔复合支架生物相容性好,是一种性能良好的骨组织工程支架材料。Objective To examine the biocompatibility of a novel nano-hydroxyapatite/poly lactic-co-glycolic acid （nHA/PLGA） composite and evaluate it feasibility as a scaffold for bone tissue engineering. Methods Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell proliferation was assessed by MTF assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted fluorescence microscope and scanning electron microscope. The cell cycle profile was analyzed by flow cytometry. Results The proliferation of the chondrocytes on the scaffold （group A） was not affected by nHA/PLGA comparing with the blank control group （group A） as it was shown by MTT assay （P〉0. 05）. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between group A and B （P〉0.05）. Conclusion The porous nHA-PLGA composite is a biocompatible and good kind of bone tissue engineering scaffold.

关 键 词：纳米复合支架 软骨细胞 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]