高尔基蛋白体73作为原发性肝癌标志物的临床应用研究  

Study of clinical application of serum Golgi protein 73 as the hepatocellular carcinoma marker

作　　者：陈琳[1] 曹亚丽[1] 吴玉兰[1] 罗文明[1] 吴月平[1] 邵建国[1] 

机构地区：[1]江苏省南通市第三人民医院检验科,南通226006

出　　处：《南通大学学报（医学版）》2013年第4期273-275,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省南通市科技局资助项目(HS2011058)

摘　　要：目的：评价高尔基体蛋白73（Golgi protein 73,GP73）作为原发性肝癌（primary hepatic carcinoma,PHC）血清学标志物的临床应用价值。方法：分别采用酶联免疫吸附试验和化学发光法定量检测PHC患者血清GP73及甲胎蛋白（alpha-fetoprotein,AFP）的水平,以肝硬化患者组及健康体检组作为对照。Spearman等级相关分析GP73与AFP之间的相关性;ROC曲线分析二者对PHC的诊断价值;对于PHC患者,进一步分析血清GP73水平与临床病理参数之间的关系。结果：PHC组中血清GP73的水平显著高于肝硬化组及健康对照组（P〈0.05）;PHC患者血清GP73与AFP具有一定的相关性（r=0.6567,P〈0.01）。血清GP73诊断PHC的曲线下面积为0.951,95%置信区间为0.916~0.986;其诊断灵敏度为66.7%,特异性为93.3%;血清AFP诊断PHC的曲线下面积为0.898,95%置信区间为0.843~0.953;其诊断灵敏度为70.6%,特异性为89.3%。病变累及大小≥3 cm的PHC患者血清GP73含量明显高于病变累及大小〈3 cm的患者（P〈0.05）;临床分期为Ⅲ＋Ⅳ的患者血清GP73含量明显高于临床分期为Ⅰ＋Ⅱ的患者（P〈0.05）;而PHC患者血清GP73水平与年龄、性别及病理分级等因素无关（P〉0.05）。结论：作为新的血清标志物,GP73与AFP一样,可用于PHC诊断,并且对于PHC病情分析、恶性程度判断乃至预后评估等具有重要临床价值。Objective： To evaluate the clinical application value of serum Golgi protein 73（GP73） as the primary hepatic carcinoma（PHC）.Methods： Quantitative detection of serum GP73 and alpha-fetoprotein（AFP） levels in patients with PHC by using enzyme-linked immunosorbent assay and chemiluminescence method,respectively,patients with cirrhosis of liver as a control and healthy examination people as another control.Spearman correlation analysis was used to analyze the level of correlation between GP73 and AFP.The value of GP73 and AFP in the diagnosis of PHC was evaluated by ROC curve analysis.To patients with PHC,the relationship between serum GP73 levels and the clinical pathologic parameters was further analyzed.Results： The serum level of GP73 in patients with PHC was significantly higher than that in patients with cirrhosis and healthy controls,P〈0.05.Serum GP73 and AFP had certain correlation in patients with PHC（r=0.6567,P〈0.01）.The area under the curve of serum GP73 in the diagnosis of PHC was 0.951 and 95% confidence interval was 0.916-0.986,the sensitivity was 66.7% and specificity was 93.3%.The area under the curve of serum AFP in the diagnosis of PHC curve was 0.898 and 95% confidence interval was 0.843-0.953,the sensitivity was 70.6% and specificity was 89.3%.Serum GP73 in patients with lesions ≥3 cm was significantly higher than that in patients with lesions 3 cm（P〈0.05）.Serum GP73 in patients with clinical stage Ⅲ＋ Ⅳ was significantly higher than that in patients with clinical stage Ⅰ＋Ⅱ（P〈0.05）.However,there was no relationship between serum GP73 and age,gender and pathologic grading in patients with PHC（P〈0.05）.Conlusion： As a new serum marker,GP73,as well as AFP,can be used for diagnosis of PHC.Moreover,it has important clinical value in analyzing pathogenetic condition,judging malignant degree and prognosis evaluation for PHC.

关 键 词：原发性肝癌 高尔基体蛋白73 肿瘤标志物 临床应用 

分 类 号：R735.7[医药卫生—肿瘤]