连翘不同部位中连翘苷和连翘酯苷A的含量分析及其入药探讨  

Analysis of the Contents of Forsythin and Forsythiaside A in Different Parts of Forsythia suspensa and Its Medicinal Use Discussion

作　　者：王进明 范圣此 李安平 陈婷婷 孙斌 杨霞 

机构地区：[1]山西振东道地药材开发有限公司,山西长治047100

出　　处：《中国现代中药》2013年第7期556-559,共4页Modern Chinese Medicine

基　　金：国家"十二五"科技支撑项目(2011BA107B01);山西省科技攻关项目(20120313015-9);长治市科技计划项目(20118033-1)

摘　　要：目的：通过连翘不同部位的连翘苷和连翘酯苷A的含量比较，为连翘资源的综合开发利用提供科学依据。方法：采用HPLC测定连翘果实、叶、果梗、枝梗中连翘苷和连翘酯苷A的含量，以Luna5uC18（2）100A（250 mm×4.6 mm，5 μm）为色谱柱，连翘苷测定流动相为乙腈-水（25∶75），检测波长为277 nm，柱温为室温，流速为1.0 mL·min-1；连翘酯苷A测定流动相为乙腈-0.4%冰醋酸溶液（15∶85），检测波长为330 nm，柱温为室温，流速为1.0 mL·min-1。结果：连翘苷在3.33～13.32 μg线性关系良好（r=0.999 7），平均回收率为99.30%，RSD=1.4%；连翘酯苷A在4.17～6.69 μg具有良好的线性关系（r=0.999 7），平均回收率为98.26%，RSD=1.8%。结论：连翘苷含量在不同部位中叶子最高，其次为果实、枝梗、果梗；连翘酯苷A含量在不同部位中同样叶子最高，其次为果实、果梗、枝梗。Objective ： To provide theoretical basis for comprehensive development and utilization of Forsythia suspensa resources by evaluating the contents of forsythin and forsythiaside A in different parts of Forsythia suspense. Methods： HPLC method was used for analyzing the contents of forsythin and forsythiaside A for 4 samples of different parts of Forsythia suspense. Luna5uC17（2） 100A （250 mm ×4. 6 mm,5 μm） Column was carried out,the mobile phrase of forsythin determination was acetonitrile-water （ 25 ： 75 ）, the detection wavelength was 277 nm. The mobile phase of forsythiaside A determination was acetonitrile -0. 4% acetic acid （15：85） and the detection wavelength was 330 nm. The column temperature was room temperature. And the flow rate was 1.0 mL. min-I Results： There was a good linear relationship （r = 0. 999 7 ） for forsythin at 3.33 - 13.32 μg, the average recovery rate was 99. 30% ,and RSD was 1.4%. The good linear relationship （r =0. 999 7） for forsythiaside A was at 4. 17 -6. 69 μg,the average recovery rate was 98.26% ,and RSD was 1.8%. Conclusion： The highest content of forsythin was in leaves, followed by fruit and branches, the highest content of forsythiaside A was in leaves, and followed by fruits, stems and branches.

关 键 词：连翘 连翘苷 连翘酯苷A HPLC 

分 类 号：R286.11[医药卫生—中药学]