银杏黄酮糖苷醇提工艺参数优化及体外抗氧化研究  

Optimization of parameters for ethanol extraction of flavone glycosides from ginkgo cell and antioxidant activity in vitro

作　　者：邵菊芳[1] 金黄涛[1] 朱红威[1] 刘炯天[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221116

出　　处：《中国矿业大学学报》2013年第4期663-669,共7页Journal of China University of Mining & Technology

基　　金：国家自然科学基金项目(21206190);中国矿业大学理科专项基金项目(LKHX04);中国矿业大学青年基金项目(OH060109)

摘　　要：为了提高银杏细胞黄酮糖苷的提取得率,以人工培养细胞为对象,利用响应面法(RSM)优化了醇法提取工艺.在单因素基础上,采用Design Expert 8.0软件中的Plackett-Burman设计,筛选出显著因素为:提取时间、提取温度和乙醇体积分数,并拟合得到二次多项式模型.细胞黄酮最佳提取条件为:提取时间3.285h、提取温度76.85℃、乙醇体积分数72.05%.在此提取条件下,利用高效液相色谱法(HPLC)验证总黄酮糖苷提取得率为1.810 1%,与模型预测值一致,说明利用RSM优化提取条件,建立的模型预测银杏细胞黄酮糖苷准确可靠,重复性好.体外抗氧化实验结果表明:黄酮糖苷能较好地清除羟自由基,抑制减缓植物油脂的自氧化过程.For improving the yield of flavone glycosides from cultured Ginkgo biloba cell,the optimization of the ethanol extraction was performed with response surface methodology（response surface methodology,RSM）.Results indicate that extract time,temperature and ethanol content have significant effects on extraction rate on the basis of univariate experiments determined by Plackett-Burman design of software Design Expert 8.0.Three factors above can be fitted into a second-order polynomial model.The best combinations achieved are with 72.05% ethanol and water for 3.285 h at 76.85℃.The yields of flavone glycosides experimentally with HPLC is 1.8101%,which is identical with the predictive value.The establishment of the model provides adequate accuracy and repeatability in predicting the yield of flavone glycosides from ginkgo cell.The results of antioxidant experiments in vitro show that the clearance of free radical and inhibition of selt-oxidant of plant oil is ideal.

关 键 词：响应面 黄酮糖苷 HPLC 

分 类 号：S567.1[农业科学—中草药栽培]