低温胁迫下2种樟树叶片超微结构的比较  

Comparison of leaf blade ultra-structures of two camphor varieties under cold stress

作　　者：王宁[1] 董莹莹 苏金乐[3] 

机构地区：[1]河南科技大学林学院,河南洛阳471000 [2]洛阳市园林局,河南洛阳471002 [3]河南农业大学林学院,河南郑州451002

出　　处：《西北农林科技大学学报（自然科学版）》2013年第7期106-112,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省重点科技攻关计划项目(0624070035)

摘　　要：【目的】探讨并比较猴樟(Cinnamonum bodinieri Levl)和芳樟(C.camphora L.(linalool-type))在低温胁迫过程中叶肉细胞超微结构的变化,揭示樟树低温胁迫的响应机制,为北方地区耐寒樟树的引种提供理论依据。【方法】以猴樟和芳樟的1年生幼苗为试材,采用室内低温处理的方法,对2种樟树在0℃下处理12,24,48h及在-5,-10℃下处理24h,取样并采用透射电子显微镜观察樟树叶肉细胞超微结构的变化。【结果】低温胁迫对2种樟树叶肉细胞超微结构均产生了一定影响。适当低温胁迫,导致2种樟树叶肉细胞器中叶绿体结构首先发生膨大变形,淀粉粒出现降解现象,液泡内含物增加。随着胁迫时间的延长和温度的降低,过度的低温胁迫导致叶绿体结构分解,淀粉粒消失,甚至出现质壁分离、细胞器崩溃降解现象。相同低温条件下,猴樟叶绿体结构遭到破坏的程度及淀粉粒降解速度均小于芳樟,至-10℃胁迫24h时,芳樟叶绿体、细胞核等器官均已遭到严重损伤,而猴樟细胞结构尚能维持完整。【结论】低温胁迫下,猴樟叶肉细胞中叶绿体等细胞器的稳定性大于芳樟,其对低温的适应能力相对较强。【Objective】 The ultra-structure changes of Cinnamonum bodinieri Levl and C.camphora L.（linalool-type） leaves cells under different artificial low temperatures were explored.【Method】 One-year-old seedlings of two camphor varieties were probed into in the condition of 0 ℃ for 12,24,and 48 hours,and-5 ℃ and-10 ℃ within for hours before the ultra-structure changes of these leaves cells were compared using the transmission electron microscope.【Result】 The results showed that ultra-structures of mesophyll cells of two camphor varieties changed obviously after low-temperature stress.The shape of chloroplasts changed firstly,starch grains decomposed slightly and cell inclusion increased in the low-temperature stress.C.bodinieri Levl had more stable chloroplasts and later decomposed starch grains might be due to its greater capacity for cold tolerance than linalool-type under low-temperature stress.After 24 hours stress of-10 ℃,cell organelles of linalool-type were damaged badly,but the cell structure of C.bodinieri Levl remained undamaged.【Conclusion】 The stability of mesophyll cells such as chloroplast organelles was greater than that of C.camphora L.（linalool-type） under the same low temperature conditions.Thus,its ability to adapt to the low temperature was stronger.

关 键 词：低温胁迫 超微结构 樟树 

分 类 号：S792.23[农业科学—林木遗传育种] Q944.56[农业科学—林学]