顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义  

Expression changes and significance of miR-27a in cisplatin-induced apoptosis of human lung adenocarcinoma cell line SPC-A1

作　　者：朱善军 韩月 徐娟 谢而付 陈丹 张美娟 张燕 娄鉴芳 曹艳 许雨乔 孙瑞红 王芳[1] 潘世扬 

机构地区：[1]南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2013年第7期873-876,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(81272324,81000754,81101322,81201359);江苏省实验诊断学重点实验室基金(XK201114);江苏高校优势学科建设工程资助

摘　　要：目的:检测miR-27a在顺铂(DDP)体外诱导人肺腺癌细胞株SPC-A1凋亡过程中的表达变化,探讨其与细胞凋亡之间的相关性及其检测的临床意义。方法:体外培养SPC-A1细胞,实验组加入终浓度为2.5μg/ml DDP,同时设置不加DDP的对照组。观察12、24、48和72 h后显微镜下细胞形态学变化,流式细胞术检测细胞凋亡率,real-time PCR检测培养上清液中和SPC-A1细胞内miR-27a的表达变化。结果:DDP(2.5μg/ml)组SPC-A1细胞形态呈凋亡改变,在48和72 h凋亡率分别为(59.3±2.5)%和(76.4±3.1)%,均显著高于对照组,差异有统计学意义(P<0.05)。DDP(2.5μg/ml)组培养上清液中和SPC-A1细胞内miR-27a含量在24、48和72 h均显著高于对照组,差异有统计学意义(P<0.05)。DDP(2.5μg/ml)组培养上清液中miR-27a含量在12、24、48和72 h逐渐升高,两两比较差异均有统计学意义(P<0.05)。结论:miR-27a在DDP诱导的SPC-A1细胞凋亡中表达升高,提示其可能参与细胞凋亡调控;对miR-27a进行定量检测有望用于对肺腺癌化疗疗效的评估。Objective：To investigate the expression changes of miR-27a in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto,and to discuss its relationship with cell apoptosis and clinical significance.Methods：SPC-A1 cells were cultured in vitro and treated with DDP at 2.5 μg / ml for 12,24,48 and 72 h.Cell morphology was observed by phase contrast microscope,as well as cell apoptosis was detected by flow cytometry;expression changes of miR-27a both in cultural supernatants and SPC-A1 cells were analyzed by real-time PCR.Results：SPC-A1 cells showed morphological changes of apoptosis in the DDP（2.5 μg / ml） group.The apoptotic rates of SPC-A1 cells at 48 and 72 h in the DDP（2.5 μg / ml） group[（59.3 ± 2.5）% and（76.4 ± 3.1）%, respectively] were higher than that in the control group（P 0.05）.PCR analysis demonstrated that the levels of miR-27a were significantly increased in the DDP（2.5 μg / ml） group both in cultural supernatants and SPC-A1 cells compared to the level of the control group（P 0.05）.Conclusion：The level of miR-27a raised in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto.Quantitative analysis of miR-27a may have important value for chemotherapy monitoring of lung adenocarcinoma.

关 键 词：顺铂 肺癌SPC-A1 细胞凋亡 miR-27a 

分 类 号：Q786[生物学—分子生物学]