miR-451及MIF在大肠癌和大肠腺瘤中的表达及临床意义  

Expression and clinical significance of miR-451 and MIF in colorectal cancer and colorectal adenoma

作　　者：汪伟[1,2] 孙晓娜[1,2] 张玉峰[1,2] 党中勤[1,2] 牛学恩[1,2] 刘晓峰[3] 

机构地区：[1]河南省中医院 [2]河南中医学院第二附属医院肝胆脾胃病科,河南郑州450000 [3]济南军区总医院消化内科,山东济南250003

出　　处：《南京医科大学学报（自然科学版）》2013年第7期882-886,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：河南省科技攻关项目(122102310614);郑州金水区技术研究与开发经费支持项目(金科[2012]23号)

摘　　要：目的:检测大肠癌及大肠腺瘤组织中miR-451及巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)的表达水平,并分析其与大肠癌相关临床病理因素的关系。方法:收集25对大肠癌组织和大肠癌旁组织,10例大肠腺瘤组织,应用RT-PCR方法检测miR-451的表达水平(以U6为对照),应用免疫组化检测MIF蛋白的表达情况,并分析二者与大肠癌相关临床病理因素的关系。结果:miR-451在大肠癌及大肠腺瘤组织中明显下调(P<0.01,P<0.05)。其中在大肠癌组织中上调6例,下调19例,下调比率76%(19/25)。在大肠腺瘤组织中上调3例,下调7例,下调比率70%(7/10)。MIF蛋白在大肠癌组织中阳性表达18例,阳性率72%,在大肠腺瘤组织中阳性表达5例,阳性率50%;癌旁组织阳性表达8例,阳性率32%,差异有统计学意义(P<0.05),并且miR-451与MIF表达水平与大肠癌的分化相关(P<0.05),与年龄、性别、Dukes分期、组织类型、浸润深度、淋巴结转移等无明显相关。结论:miR-451在大肠癌组织及大肠腺瘤组织中低表达,可能通过抑制MIF负向调控了大肠癌的发生和发展。Objective：To investigate the expression of miR-451 and MIF in colorectal cancer and colorectal adenoma and their correlation with clinicopathologic features in colorectal cancer.Methods：Real time polymerase chain reaction（RT-PCR） was performed to examine the expression level of miR-451 in 25 cases of colorectal cancer and matched non tumor adjacent tissue and 10 cases of colorectal adenoma tissue specimens.MIF protein was detected by immunohistochemistry（SP method）.The relationships among the expression of miR-451,MIF and the clinicopathologic features of colorectal cancer were analyzed.Results：The expression level of miR-451 was significantly lower in the tumor tissues and adenoma tissues than that in the adjacent tissues（P 0.01,P 0.05）.There were 6 cases up-regulated and 19 cases down-regulated in colorectal cancer,and the down-regulated rate was 76%（19 / 25）.There were 3 cases up-regulated and 7 cases down-regulated in colorectal adenoma,and the down-regulated rate was 70%（7 / 10）.The positive rate of MIF in colorectal cancer was 72%,the positive rate was 50% in colorectal adenoma and the positive rate was 32% in adjacent tissues.Down-regulated miR-451 expression and up-regulated MIF were associated with the cell differentiation（P 0.05） in colorectal cancer patients.No significant association was found between the expression of miR-451 and MIF with the status of age,gender,Dukes staging system histological type,depth of tumor invasion and lymphatic metastasis.Conclusion：Up-regulated miR-451 expression may inhibit MIF expression,and then control colorectal carcinogenesis and the development process.

关 键 词：大肠癌 大肠腺瘤 微小RNA miR-451 

分 类 号：Q786[生物学—分子生物学]