鸡卵清蛋白上游启动子转录因子Ⅱ在人胃癌组织中的表达及临床意义  

Expression and clinical significance of chicken ovalbumin upstream promoter transcription factor Ⅱ protein in human gastric carcinoma tissues

作　　者：李增亮[1] 姜宝飞[1,2] 李伟[1] 徐皓[1] 徐泽宽[1] 

机构地区：[1]南京医科大学第一附属医院胃肠外科,江苏南京210029 [2]南京医科大学附属淮安市第一人民医院胃肠外科,江苏淮安223001

出　　处：《南京医科大学学报（自然科学版）》2013年第7期892-896,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(81072031)

摘　　要：目的:检测鸡卵清蛋白上游启动子转录因子Ⅱ(COUP-TFⅡ)在人胃癌组织中的表达,并探讨其临床意义及其影响胃癌发生、发展的可能分子机制。方法:收集66例手术切除的胃癌组织和相对应的癌旁非肿瘤组织标本,采用免疫组织化学法检测标本中COUP-TFⅡ和基质金属蛋白酶-2(MMP-2)蛋白的表达,分析COUP-TFⅡ与患者临床病理特征及预后之间的关系,以及COUP-TFⅡ与其潜在靶基因MMP-2的相关性。结果:COUP-TFⅡ蛋白在胃癌和癌旁组织中的阳性表达率分别为65.2%(43/66)和19.7%(13/66),两者比较差异有统计学意义(P<0.05)。COUP-TFⅡ蛋白的表达与胃癌的浸润深度、分化程度、淋巴结转移及TNM分期密切相关(P<0.05),而与患者的性别、年龄、肿瘤大小无关(P>0.05)。COUP-TFⅡ蛋白阳性表达的患者术后平均生存时间比阴性表达者短,分别为(26.8±2.6)个月和(36.4±3.0)个月(χ2=4.118,P=0.042)。MMP-2蛋白在胃癌和癌旁组织中的阳性表达率分别为78.8%(52/66)和34.8%(23/66),两者比较差异有统计学意义(P<0.05)。COUP-TFⅡ与MMP-2蛋白在胃癌组织中的表达呈正相关(r=0.310,P=0.011)。结论:COUP-TFⅡ蛋白与胃癌的发生、发展、侵袭、转移以及不良预后密切相关,推测可能是通过调控MMP-2的表达而发挥作用。Objective：To detect the expression of chicken ovalbumin upstream promoter transcription factor Ⅱ（COUP-TF Ⅱ） protein in human gastric cancer（GC） tissues,and investigate the potential molecular mechanism of COUP-TF Ⅱ involved in the development and advancement of GC and its clinical significance.Methods：Paired tumorous and adjacent non-tumorous human gastric tissues were obtained from 66 patients with GC who underwent surgical resection.The expressions of COUP-TFⅡ and MMP-2 protein were detected using immunohistochemistry.The relationship between COUP-TFⅡ and the clinicopathologic factors as well as the correlation of COUP-TFⅡ and MMP-2 were analyzed.Results：COUP-TFⅡ proteins were positively expressed in 65.2%（43 / 66） and 19.7%（13 / 66） of GC tissues and paired non-GC tissues,respectively.The expression of COUP-TFⅡ was significantly correlated with the depth of invasion,differentiated grade,lymph node metastasis and tumor-node-metastatsis（TNM） stage（P 0.05）.However, the expression of COUP-TFⅡ was not correlated with the gender,age and volume of GC tissues.Patients with positive COUP-TFⅡ expression had lower mean survival time than those with negative COUP-TFⅡ expression（26.8 ± 2.6 months vs 36.4 ± 3.0 months, χ 2 = 4.118,P = 0.042）.MMP-2 proteins were positively expressed in 78.8%（52 / 66） and 34.8%（23 / 66） of GC tissues and paired non-GC tissues,respectively.Moreover,a positive correlation was found between COUP-TFⅡ and MMP-2 expression in GC tissues. Conclusion：COUP-TF Ⅱ protein has significant value in determining invasion and metastasis of gastric cancer and assessing prognosis in patients with gastric cancer,and MMP-2 may be involved in the above regulation progress.

关 键 词：胃癌 鸡卵清蛋白上游启动转录因子Ⅱ 基质金属蛋白酶-2 免疫组织化学 

分 类 号：Q786[生物学—分子生物学]