毛冬青甲素对红白血病细胞K562凋亡相关基因survivin和bax表达机理的研究  

Mechanism Research of Ilexonin A on Erythroleukemia Cell K562 Apoptosis Related Gene Expression of Survivin and Bax

作　　者：赵早云[1] 朱惠斌[1] 席建元[1] 江劲波[1] 周欣欣[1] 

机构地区：[1]湖南中医药大学第一附属医院,长沙410007

出　　处：《光明中医》2013年第3期477-480,共4页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：湖南省科学技术厅科技计划项目(No:2010FJ3021)

摘　　要：目的探讨毛冬青甲素对红白血病K562细胞凋亡的表达机制。方法 MTT比色法测定细胞增殖的抑制率;电镜下观察细胞凋亡形态变化;RT-PCR法和流式细胞术(FCM)检测毛冬青甲素对K562细胞survivin和bax表达影响。结果毛冬青甲素对K562细胞生长有抑制作用,并呈剂量一时间效应关系。毛冬青甲素处理细胞后可见凋亡形态学变化,细胞核染色质浓缩,电子密度增加,细胞质内出现空泡化。RT-PCR法和FCM检测结果显示毛冬青甲素可抑制survivin上调,促进bax的表达。结论毛冬青甲素能诱导K562细胞凋亡,其机制可能与调节survivin和bax的表达有关。Objective To explore Ilexonin A on erythroleukemia K562 apoptosis expression mechanism. Method MTT colorimetric method determination of cell proliferation inhibition rate; By electron microscopy to observe apoptosis morphology change; RT-PCR method and flow eytometry （FCM） detection llexonin A on K562 ceils survivin and bax expression effect. Results pubescent Ilexonin A on K562 cell growth have inhibition, and the dose effect relationship at that time. Ilexonin A processing cells visible apoptosis morphology change, part of the cell volume smaller, chromatin concentrated and edge set. RT-PCR method and FCM detection results show that ilexohin meal can inhibit survivin rise, promote bax expression. Conclusion pubescent Ilexonin A can induce apoptosis of K562 cells, whose mechanism is probably and regulating the expression of survivin and bax relevant. Apoptosis; survivin;bax

关 键 词：毛冬青甲素 红白血病 K562细胞 细胞凋亡 SURVIVIN bax 

分 类 号：R285.5[医药卫生—中药学]