异位子宫内膜间质细胞分泌催乳素的体外研究  

In Vitro Study of Prolactin Secretion by Ectopic Endometrial Stromal Cells

作　　者：郝敏[1] 石一复[1] 周彩云[1] 

机构地区：[1]浙江大学医学院附属妇产科医院妇科,杭州 310006

出　　处：《中华妇产科杂志》2000年第9期527-529,I010,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨异位子宫内膜间质细胞分泌催乳素 (PRL)与子宫内膜异位症 (内异症 )患者腹水中高PRL水平的关系。方法　对 12例内异症患者的异位内膜及 12例正常育龄妇女的在位子宫内膜进行体外细胞分离、培养。两种内膜间质细胞在加入 10 -8mol/L浓度孕酮培养 6d后 ,用酶联免疫吸附试验法测定其分泌的PRL水平 ;并对异位内膜间质细胞分泌PRL水平与美国生育协会 (AFS)修正标准分期评分进行相关性分析。采用免疫细胞化学法检测PRL蛋白在两种间质细胞中的表达。结果　异位及在位内膜间质细胞分泌相似量的PRL ,分别为 1× 10 5个细胞 (2 1.8± 8.0 )和 (2 4.5± 7.9)μg·L-1·6d-1(P >0 .0 5 ) ,异位内膜间质细胞分泌的PRL与AFS评分呈正相关 (P <0 .0 5 )。免疫细胞化学染色 ,PRL在两者的积分分别为 4.1± 0 .8和 4.3± 0 .9(P >0 .0 5 )。Objective To investigate the relationship between prolactin (PRL )secretion by ectopic endometrial stromal cells and elevated PRL concentrations of peritoneal fluid in patients with endometriosis. Methods Twelve samples of each ectopic endometrium and normal endometrium were separated and cultured in vitro. After stimulated with progesterone(10 -8 mol/L) for 6 days, PRL levels in the media of cultured stromal cells from both tissue types were measured by enzyme labeled immunosorbent assay(ELISA). A study of correlation between PRL level in culture supernatant of stromal cells from ectopic endometrium and American Fertility Society(AFS) classification of endometriosis score was performed. The expression of PRL protein of stromal cells from both tissue types were determined by immunocytochemical method. Results PRL was secreted in similar concentrations by stromal cells from both tissue types. The mean levels of PRL in ectopic and normal endometria were (21.8 ±8.0) and (24.5±7.9) μg·L -1 ·6 d -1 per, respectively ( P >0.05). There is significant positive correlation between PRL secretion by ectopic endometrial stromal cells and the scoring of endometriosis ( P < 0.05) . There were no significant difference in the immunostaining integral score of PRL between the two groups. Conclusion Production of PRL by ectopic implants of endometriosis is likely to be a contributing factor of the elevated peritoneal fluid PRL in patients with endometriosis.

关 键 词：子宫内膜异位 间质细胞 细胞培养 催乳素 

分 类 号：R711.7[医药卫生—妇产科学]