不同浓度铁离子对栅藻生理生化特性和生长的影响  

Effect of Ferric Concentration on the Growth and Physiological Biochemical Characteristics of Scenedesmus sp.

作　　者：周新虹[1] 占凌云[1] 黄建军[1] 王明兹[1] 李敏[1] 陈必链[1] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心,生命科学学院,福建省现代发酵技术工程研究中心,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2013年第4期114-119,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省发展与改革委员会资助项目(闽发改高技[2011]1598);福建省环境保护厅资助项目(FJHK200912)

摘　　要：研究了不同浓度铁离子对栅藻生长和藻细胞抗氧化系统的影响.分析测定了培养基初始Fe3+分别为0,1.2×10-3,1.2×10-4,1.2×10-5,1.2×10-6,1.2×10-7mol.L-1时栅藻的细胞密度、生物量、蛋白质含量、总脂产量及脂肪酸组成,并研究了不同浓度铁离子对栅藻细胞的抗氧化系统的影响.实验结果表明,当培养基中初始铁离子浓度为1.2×10-4mol.L-1时最适合栅藻生长和蛋白质合成,蛋白质含量达到最大为97.33μg.mL-1;当初始Fe3+浓度为1.2×10-5mol.L-1时,栅藻细胞总脂占干质量最高达38.60%;C18∶2和C18∶3占总脂肪酸的比例随Fe3+浓度增加而降低.当Fe3+浓度达到1.2×10-3mol.L-1时,栅藻的生长受到抑制.与对照组(培养基不添加铁离子)相比,不同浓度铁离子使栅藻总抗氧化能力增加,超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)活性有不同程度的提高,羟自由基能力受到不同程度的抑制.The effects of Fe3 ＋ on the growth and physiology of Scenedesmus sp. were investigated. Fe^3＋ was added at the concentration of 0, 1.2×10^-3, 1.2×10^-4, 1.2×10^-5, 1.2×10^-6 and 1.2× 10^-7 mol · L^-1. The results showed that the presence of low concentration of Fe^3＋ en- hanced the growth of Scenedesmus sp. , the inhibition was found at a high Fe^3＋ concentration of 1.2 × 10^-3 mol · L^-1. When the concentration of Fe3＋ reached 1.2 ×10^-4 mol · L^-1, it was most available for Scenedesmus sp. ＇s growth and the synthesis of protein which highest content was up to 97.33 μg · mL^-1. The total lipid content accounted for the highest percentages of cell dry weight, was up to 38.6%, in the medium with 1.2 x 10-5 tool ~ L-1 Fe3＋. The ratio of C18：2 and C18： 2 to total lipid increases as Fe^3＋ contents decrease. With different concentrations of Fe^3＋ added, the activities of antioxidase in Scenedesmus sp. were higher than those in the control group.

关 键 词：栅藻 三价铁离子 抗氧化系统 生长 

分 类 号：P735[天文地球—海洋生物学]