牛晶状体上皮细胞在不同材料人工晶状体表面生长的比较研究  

Adherence of bovine lens epithelial cells to the surface of intraocular lens made of different materials

作　　者：吴仁毅[1] 姚克[1] 孙朝晖[1] 徐雯[1] 陈佩卿[1] 

机构地区：[1]浙江大学医学院附属第二医院眼科中心,杭州310009

出　　处：《中华眼科杂志》2000年第5期348-350,I022,共3页Chinese Journal of Ophthalmology

基　　金：卫生部科学研究基金资助项目!(981333);浙江省医药科技基金资助项目!(98A034)

摘　　要：目的　研究和比较体外牛晶状体上皮细胞 (bovinelensepithelialcell,BLEC)在聚甲基丙烯酸甲酯 (polymethylmethacrylate ,PMMA)、硅胶 (silicone ,SI)、水凝胶 (hydroxyehtylmethacrylate ,HEMA)和丙烯酸酯 (acrylic ,acrysof;ACRY)人工晶状体 (intraocularlens,IOL)表面生长的情况 ,为临床选用IOL提供参考。方法　体外将BLEC接种到 4种IOL表面培养 ,用流式细胞仪对其表面生长的细胞进行计数分析。结果　细胞计数显示 4种IOL表面BLEC生长数量不同 ,ACRY的IOL最多 ,HEMA的IOL最少 ,SI和PMMA的IOL介于中间且两者差异无显著性 (P >0 0 5 )。结论　不同材料IOL表面性能影响晶状体上皮细胞粘附和生长 。Objective To compare the in vitro adherence of bovine lens epithelial cells (BLEC) onto the surface of intraocular lens (IOL) made of 4 different materials: polymethylmethacrylate (PMMA), silicone (SI), hydroxyethylmethacrylate (HEMA) and acrylic (Acrysof, ACRY) Methods BLEC were incubated with IOLs four 48 hours Adherent cells were counted by flow cytometry technique Results The amount of the adherent cells on IOLs of ACRY was the highest, that of HEMA the least, and there was no significant difference between the amount on PMMA and SI IOLs (P>0 05) Conclusions Adherence of lens epithelial cells to IOLs is influenced by the surface property of IOL materials, which may influence the occurrence of postoperative capsular opacification after IOL implantation

关 键 词：人工晶体 白内障 细胞粘附 牛 晶状体上皮细胞 

分 类 号：R318.0[医药卫生—生物医学工程]