基于Illumina高通量测序的泡桐转录组研究  

Illumina-Based de novo Sequencing and Characterization of the Transcriptome of Paulownia Plant

作　　者：邓敏捷[1,2] 董焱鹏 赵振利[1,2] 张晓申[1,3] 范国强[1,2] 

机构地区：[1]河南农业大学泡桐研究所,郑州450002 [2]河南农业大学林学院,郑州450002 [3]郑州市农林科学研究所,郑州451400

出　　处：《林业科学》2013年第6期30-36,共7页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30271082;30571496);高等学校博士学科点专项科研基金项目(20050466003);河南省杰出人才计划项目(122101110700)

摘　　要：利用新一代高通量测序技术平台Illumina Solexa对泡桐进行转录组测序和数据denovo组装,并对得到的unigene进行功能注释、分类及代谢通路分析。结果表明,N50值为2278bp、平均长度为1410bp的泡桐unigene共有188019条,将其与NCBI的Nr和Swiss-Prot数据库比对后发现,分别有120808条和85880条unigene与其他物种的基因具有同源性。利用COG数据库可将有关的41914条泡桐unigene分成25类,KEGG数据库分析发现共有43553个unigene参与215种代谢通路。找到与木质素合成相关的泡桐unigene。The transcriptome Paulownia plant was sequenced by using Illumina technology, and the functional annotations and metabolic pathways analyses of some unigenes were conducted in this present paper. The results indicated that 188 019 unigenes with an average length of 1 410 bp and N50 value of 2 278 bp were obtained with de novo assembly. Comparison with NCBI and Swiss-Prot protein databases showed that 120 808 and 85 880 unigenes had homology with the genes from other species, respectively. The 41 914 unigenes were able to be assigned to 25 categories with the COG （Clusters of Orthologous Groups） database. Functional annotation against KEGG （Kyoto Encyclopedia of Genes and Genomes） database identified 43 553 unigenes which were mapped to 215 metabolic pathways. Moreover, some Paulownia unigenes related to lignin biosynthesis were found using this information platform. The result provides reference for future excavations of new genes, as well as screening and identifying miRNA of Paulownia plants.

关 键 词：泡桐 转录组 Illumina高通量测序 从头组装 

分 类 号：S718.46[农业科学—林学]