柳蛎盾蚧危害与丁香不同种(品种)间叶片单宁、酚酸含量的关系  

Relationships between Lepidosaphes salicina Attack and Contents of Tannins and Phenolic Acids in Leaves of Different Syringa Species and Cultivars Leaves

作　　者：程红[1] 严善春[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《林业科学》2013年第6期97-106,共10页Scientia Silvae Sinicae

摘　　要：根据柳蛎盾蚧在丁香上的虫口密度,应用聚类分析法将13种(品种)丁香分为高抗类(什锦丁香等7种)、中抗类(西南丁香等3种)、易感类(紫丁香等2种)、高感类(红丁香)。分别在柳蛎盾蚧的未危害期(5月末)、危害盛期(6月末)、危害弱期(7月末)、危害末期(8月末)采集叶片,用香兰素盐酸法、高效液相色谱法测定其内的单宁、酚酸含量,分析丁香叶中单宁、总酚酸含量、酚酸种类等与柳蛎盾蚧危害的关系。结果表明:高抗类丁香叶中的酚酸种类齐全,或者未危害期内总酚酸、单宁含量均显著高于其余种(品种)(P<0.05),或者危害盛期单宁、总酚酸增幅均显著高于其余种(增幅为81.75%～888.43%)。中抗类丁香,只在危害盛期总酚酸含量增幅显著高于易感和高感类丁香,单宁含量显著高于易感类丁香。易感和高感类丁香在未危害期单宁和总酚酸含量相对较低,危害盛期单宁或总酚酸含量存在诱导滞后性,酚酸种数只是高抗和中抗类丁香的1/2。此外,一些特定酚酸种类的有无及其含量的多少也与丁香的抗性相关,如:高抗类及中抗类丁香在5—8月均检测到肉桂酸,易感和高感类丁香5—8月均未检测到肉桂酸。总之,丁香中酚酸种类多、总酚酸或单宁含量高,或者其单宁和总酚酸含量能在虫害后迅速升高、增幅大的种类,对柳蛎盾蚧的抗性就强,反之则弱。In order to study the resistance mechanism of Syringa spp. against Lepidosaphes salicina, 13 species/ cuhivars of Syringa were categorized into four groups： highly-resistant group （ including 7 species, such as Syringa chinensis） , moderately-resistant group （3 species such as S. emodi） , susceptible group （2 species such as S. oblata） , highly-susceptible group （only 1 species, e.g.S, villosa） , on the basis of the population density of L. salicina on the Syringa spp. by cluster analysis. In the present study, the leaves of the 13 species/cuhivars were sampled separately in pre-infesting period （by the end of May） , peak-infesting period （by the end of June） , weakly-infesting period （by the end of July） and late-infesting period （by the end of August） , the contents of various tannins and phenolic acids of the samples were measured by Vanillin-HC1 and HPLC methods, and the relationships between the leaf extracts and the susceptibility to L. salicina were analyzed. The results showed that in the highly-resistant group, a full range/types of phenolic acids were detected in the uninfected leaves, and the contents of both total phenolic acids and tannins in the uninfected leaves was significantly higher than those species/euhivars in other groups （P 〈 0.05） , the total phenolic acids and tannins in the infected leaves of the highly-resistant group increased by 81.75% -888.43% and the increments were higher than other groups. In the moderately-resistant group, the increment in total phenolic acids contents at the peak- infesting period was significantly higher than that in the highly-susceptible and susceptible groups, and the tannins content was significantly higher than that of susceptible group. The susceptible and highly-susceptible groups had relatively low contents of tannin and total phenolic acids during the pre-infesting period, and delayed induction of tannins and total phenolic acids during the peak-infesting period, and the types of phenolic acids were only a half

关 键 词：昆虫危害 植物次生代谢物 丁香 柳蛎盾蚧 单宁 酚酸 

分 类 号：S433.1[农业科学—农业昆虫与害虫防治] S763.41[农业科学—植物保护]