肝炎患者肝组织中庚型肝炎病毒相关抗原的表达  

The expression of hepatitis G virus-related antigens in the liver tissues of patients with hepatitis

作　　者：聂青和[1] 李梦东[2] 胡大荣[2] 李玲[2] 朱永红[2] 

机构地区：[1]第四军医大学唐都医院传染病科,西安710038 [2]第三军医大学西南医院全军传染病中心

出　　处：《中华传染病杂志》2000年第3期173-175,I003,共4页Chinese Journal of Infectious Diseases

摘　　要：目的　研究庚型肝炎病毒 (HGV)相关抗原在肝组织中定位。方法　采用免疫组织化学方法 ,用抗 HGV非结构蛋白 5 (NS5 )单克隆抗体 (McAb)对 196例肝炎患者肝组织中的HGV相关抗原进行了检测。结果　 196例肝炎患者肝组织中的HGV相关抗原表达阳性率为 2 5 .0 % ;在血清中HGVRNA呈阳性患者肝组织中抗原检出率为 38.3% ;单一感染者肝组织中抗原表达阳性率为6 6 .7% ;阳性信号位于胞浆中 ,阳性信号表达阳性细胞可位于坏死灶中或灶旁 ,碎屑样坏死处也可见该病毒抗原表达。结论　初步研究结果表明HGV相关抗原在肝损伤过程中可能起到靶抗原的作用。Objective To study the distribution of hepatitis G virus (HGV) related antigens in liver tissues and discuss the mechanisms of liver damage induced by HGV.Methods HGV related antigens were detected by immunohistochemical methods in paraffin embedded liver tissue.Immunohistochemical assay were performed using anti HGV NS5 monoclonal antibody (McAb).Results The expression of HGV related antigen was positive in 25.0% of all 196 liver samples, 38.3% of patients with serum HGV RNA positive and 66.7% of patients with HGV infection alone.The viral antigens were expressed in cytoplasm and positive cells were located at the center of necrosis foci or surrounded them. Antigen expressions could also be seen at the sites of piecemeal necrosis.Conclusion The preliminary results suggested that HGV might exert its role as a target antigen in the course of liver damage. HGV infection was common in the liver tissues of patients with chronic liver diseases.

关 键 词：抗原 免疫组织化学 单克隆抗体 庚型肝炎 HGV 

分 类 号：R512.6[医药卫生—内科学]