高效液相色谱法测定向日葵花盘中绿原酸含量  

Determination of the Contents of Chlorogenic Acid Sunflower Disc by HPLC

作　　者：索金玲[1] 阿依丁[1] 张金龙[1] 彭秧[2] 

机构地区：[1]阿拉山口出入境检验检疫局技术中心,新疆阿拉山口833418 [2]新疆大学化学化工学院,新疆乌鲁木齐830046

出　　处：《广州化工》2013年第14期138-139,154,共3页GuangZhou Chemical Industry

摘　　要：建立了向日葵花盘中药用成分绿原酸的高效液相色谱(HPLC)定量分析方法。色谱柱:Agilent XDB C18(4.6 mm×50 mm,1.8μm);流动相:乙腈:0.1%磷酸(11∶89);流速:0.5 mL/min;检测波长:328 nm。通过测定,向日葵花盘中绿原酸含量为0.227%。方法精密度RSD为0.13%,重复性良好,平均回收率为102.69%,绿原酸浓度在0.67～67μg/mL呈良好线性关系,r=0.99998。该方法稳定、可靠,可作为该药材的质量控制方法。A method of determination the content of chlorogenic acid in sunflower disc by high performance liquid chromatography （HPLC） was developed. The conditions were column of Agilent XDB C18 （4. 6 mm ×50 mm, 1.8 μm）, mobile phase： acetonitrile： 0. 1% phosphoric acid （eleven： eighty -nine）, flow rate 0.5 mL/min, and detection wave- length 328 rim. The content of chlorogenic acid in sunflower flower was 0. 227%, and the method precision （RSD） was 0. 13%, repeatability. The average recovery was 102.69%, and chlorogenic acid showed a good linear relationship between concentration 0.67 - 67 μg/mL, and r = 0. 99998. The method was stable, reliable, and could be used as a quality control method of the medicine.

关 键 词：向日葵花盘 绿原酸 高效液相色谱 

分 类 号：O657[理学—分析化学]