体细胞克隆技术在企业肉羊繁殖生产中的初步应用  

Application of Somatic Cell Nuclear Transfer Techniques in Sheep Farm

作　　者：孟蝶[1] 白春玲[1] 银凤霞[1] 苏广华[1] 魏著英[1] 程磊[1] 李俊龙 李光鹏[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室、农业部草食家畜繁殖生物技术与育种重点实验室,呼和浩特010070 [2]内蒙古赛诺草原羊业有限公司,呼和浩特010040

出　　处：《内蒙古大学学报（自然科学版）》2013年第4期404-408,共5页Journal of Inner Mongolia University：Natural Science Edition

基　　金："973"课题"克隆与转基因克隆动物机理研究"(2012CB22306);内蒙古自治区科技重大项目"优秀家畜转基因技术研发"资助

摘　　要：虽然体细胞克隆技术(somatic cell nuclear transfer,SCNT)的总体效率不高,但其潜在的开发价值仍值得尝试.本试验与企业密切合作,并以企业为开发主体,开展优秀杜泊种公羊的克隆生产与应用研究.结果表明,舍饲条件下的受体母羊比草原放养母羊更适合于作克隆胚胎移植受体,同期发情处理方式与胚胎运输影响克隆胚胎的妊娠效率.在过去3年间,共有100只受体羊妊娠至3个月以上,妊娠率为13.6%.目前有61只健康存活.其中一部分克隆公羊作为种羊与本地蒙古羊配种,参与企业的肉羊繁殖生产.由此可以看出,以企业为主体的利用克隆技术进行优秀种羊规模化生产是可行的.Although the efficiency of somatic call nuclear transfer（SCNT）,especially in farm animals is still very low,the potential applications of this technique in excellent farm animal reproduction are valued to make trials.The present study is a farm-university-research cooperation,the farm as a main body,to produce the proven excellent Dorper rams by SCNT techniques.The results show that the barn feeding recipient sheep are more suitable for cloned embryo transfer than the pastured sheep.Estrus synchronization protocols and shipment of cloned embryos affect the recipient synchronization and pregnancies.One hundred recipient ewes got pregnancy,with 13.6% pregnant rate during the last three years.Sixty-one cloned lambs are healthily survived.Most of the cloned Dorper rams are currently used in the production of sheep with local Mongolian sheep.In conclusion,this preliminary study indicates the application of SCNT techniques in sheep farm to produce the excellent sheep individuals is highly available.

关 键 词：动物克隆 家畜 规模化生产 杜泊羊 

分 类 号：S813.3[农业科学—畜牧学]