疏肝健脾方对腹泻型肠易激综合征大鼠结肠黏膜5-羟色胺及其受体的影响  

Effects of Shuganjianpi prescription on the 5-HT and its receptors of colon mucosa in D-IBS rats

作　　者：汪正芳[1] 郭前坤[2] 张声生[1] 赵鲁卿[1] 杨成[1] 李晓玲[3] 吴震宇[3] 

机构地区：[1]首都医科大学附属北京中医医院消化中心,北京100010 [2]天津中医药大学第一附属医院脾胃病科,天津300193 [3]北京中医药大学,北京100029

出　　处：《北京中医药》2013年第6期406-409,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30973746);北京市自然科学基金(7102083);北京市教委基金(KM201010025027)

摘　　要：目的观察疏肝健脾方对腹泻型肠易激综合征(D-IBS)模型大鼠结肠黏膜5-羟色胺(5-HT),5-羟色胺3受体(5-HT3R)及5-羟色胺4受体(5-HT4R)表达的影响。方法将60只SD雄性大鼠随机分为正常组、模型组、得舒特组和疏肝健脾高、中、低剂量组,除正常组外其余各组使用乙酸灌肠加束缚应激的方法制备D-IBS大鼠模型,造模成功后各组分别予生理盐水、得舒特、疏肝健脾方灌胃2周。取大鼠结肠黏膜,采用酶联免疫法检测5-HT,免疫组化法检测5-HT3R及5-HT4R的表达。结果疏肝健脾方可以明显降低D-IBS大鼠粪便含水量(P<0.01);疏肝健脾方可以明显增加D-IBS大鼠的最小容量阈值(P<0.01);疏肝健脾方可以降低D-IBS大鼠结肠黏膜5-HT和5-HT3R的表达(P<0.01),升高5-HT4R的表达(P<0.01)。结论疏肝健脾方对D-IBS模型大鼠的治疗作用可能是通过调节结肠5-HT及其受体,减弱神经元兴奋性、提高内脏痛阈、消除肠道敏感而达到的。Objective To investigate the effects of Shuganjianpi prescription（SGJP） on the expression of 5-hydroxytryptamine（5-HT）,5-HT3receptor（5-HT3R） and 5-HT4receptor（5-HT4R） of colon mucosa in the irritable bowel syndrome-diarrhea predominant（D-IBS） rats.Methods Sixty male Sprague-Dawley（SD） rats were randomly divided into six groups： a normal group,a model group,a Pinaverium Bromide（PB） group and a high-,medium-and low-dose of SGJP group（Group H,M and L）.All groups except the normal group received enema of acetic acid and restraint stress for preparation of D-IBS model,which were intragastric administrated with saline,PB,and SGJP for two weeks respectively after modeling successfully.The colon mucosa of rats was taken for detecting the expression of 5-HT,5-HT3R and 5-HT4R by enzymelinked immunosorbent assay and immunohistochemical assay.Results The SGJP significantly reduced the fecal water content of D-IBS rats（P 0.01）;the SGJP significantly increased the minimum capacity threshold of D-IBS rats（P 0.01）;the SGJP significantly reduced the expression of 5-HT and 5-HT3R of colon mucosa in D-IBS rats（P 0.01）,while significantly increased expression of 5-HT4R（P 0.01）.Conclusion The therapeutic action of SGJP on the D-IBS rats might be realized through regulating 5-HT and its receptors,reducing neuronal excitability,increasing visceral pain threshold and eliminating intestinal sensitivity.

关 键 词：腹泻型肠易激综合征 疏肝健脾方 5-羟色胺 5-HT3受体 5-HT4受体 

分 类 号：R285.5[医药卫生—中药学]