小鼠周脂素基因的原核表达及纯化  

Prokaryotic Expression and Purification of Mouse Prilipin Gene

作　　者：卫兵艳[1] 刘田福[1] 樊林花[1] 

机构地区：[1]山西医科大学实验动物中心,太原030001

出　　处：《中国医疗前沿》2013年第11期9-10,36,共3页China Healthcare Innovation

摘　　要：目的构建小鼠周脂素(prilipin,Plin)原核表达载体,表达周脂素蛋白并进行初步纯化,为研究周脂素的功能奠定基础。方法通过酶切从pMD18-Plin重组载体上酶切下周脂素目的基因,定向插入原核表达载体pET-32a(+)中,转化大肠杆菌BL21(DE3),IPTG诱导表达。表达产物经SDS-PAGE及Western blot鉴定后,进行纯化。结果构建的pET-32a-Plin载体能够表达周脂素重组蛋白,表达量约占菌体总蛋白的50%,经初步纯化后,纯度达95%以上。结论成功构建了周脂素原核表达载体,并在原核系统中表达了重组蛋白,纯化的蛋白纯度较高,可用于后续试验。Objective To structure the prokaryotic expression vector of mouse prilipin gene,was expressed in prokaryotic cells and purify the expressed product.Methods By enzyme digestion from pMD18-Plin recombinant enzyme cut the prilipin gene.The target gene was recovered and cloned into expression vector pET-32a（＋）,and the constructed recombinant plasmid pET-32a-Plin was transformed to E.coli BL21（E3） for expression under induction of IPTG.The expressed product was identified by SDS-PAGE and Western blot,then purified by His label chromatography.Results The prokaryotic expression vector of mouse prilipin gene can express prilipin.The expressed recombinant prilipin,with a relative molecular mass of about 57 kDa,contained about 50% of total somatic protein.It showed good reactogenicity and reached a purity of more than 95% after purification.Conclusion The prokaryotic expression vector of mouse prilipin gene was successfully structured and expressed in prokaryotic cells,and the expressed product reached a high purity,which laid a foundation of study on the function of prilipin.

关 键 词：周脂素 原核表达 纯化 

分 类 号：R392.1[医药卫生—免疫学]