山东牛心柿群体遗传多样性和亲缘关系的SSR分析  

Genetic diversity and relationship analysis of oriental persimmon (Diospyros kaki) populations in Shandong by SSR

作　　者：艾呈祥[1] 秦志华[1] 王洁[1] 王长君[1] 安淼[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《果树学报》2013年第4期558-562,共5页Journal of Fruit Science

基　　金："十二五"农村领域国家科技计划项目(2013BAD14B05);山东省农业良种工程(2010lz010)

摘　　要：【目的】从分子水平了解山东牛心柿群体的遗传结构和亲缘关系。【方法】采用SSR分子标记技术对山东省内的8个牛心柿群体共207个个体进行检测,应用POPGENE 1.31软件进行遗传参数分析,采用AREQUIN 2.0软件对群体间和群体内的遗传变异进行分子变异分析,利用TFPGA软件对Nei’s无偏差预期杂合度和基因分化系数进行了统计计算。【结果】10对SSR引物共检测到128个位点,其中105个位点为多态位点,占82%。Nei’s遗传多样性指数（He）在0.153 8~0.247 6,Shannon信息指数（H0）在0.221 7~0.372 1。群体间的遗传变异占总遗传变异的24.17%（P〈0.001）,群体间的遗传距离（D〈0.231 7）、遗传一致度（I〉0.783 2）、基因分化系数（φST=0.222 6）和遗传分化系数（FST=0.175 9）均表明各群体间产生了一定程度的遗传分化。【结论】山东牛心柿群体遗传背景丰富,群体间一定程度的遗传分化可能是人为选择压力所引起。[Objective] The objective of the experiment is to understand the diversity and genetic structure of oriental persimmon （Diospyros kaki） populations in Shandong. [Method] The genetic diversity of 207 individuals from 8 persimmon （Diospyros kaki） populations in Shandong were investigated using SSR markers. The genetic parameters were analyzed using POPGENE 1.31 software, genetic variation of inter- population and inner-population were analyzed by AREQUIN 2.0 version, and then the mean expected heterozygosity （He） and genetic differentiation coefficient were counted by TFPGA software. [Result] As a result, 128 bands, of which 105 were polymorphic loci, were amplified by 10 SSR primers selected from 51 markers. The relatively high levels of PPL （82%）, NeFs gene diversity （Heranged from 0.153 8 to 0.247 6） and Shannon＇s information diversity （H0 ranged from 0.221 7 to 0.372 1） revealed relatively rich genetic diversity in the 8 persimmon populations. Analysis of molecular variance （AMOVA） showed that the vari- ance component among populations contributes 24.17% at a significantly high level （P〈0.O01）. Further- more, a low level of genetic variation among populations was detected based on the genetic distance （D〈 0.231 7）, identity （I〉0.783 2）. Neits genetic diversity analysis and AMOVA revealed that there was a higher level of genetic differentiation among populations （~sr= 0.222 6, FST= 0.175 9）. [Conclusion]The oriental persimmon populations in Shandong possess abundant genetic diversity, the genetic differentia- tion between populations may result from human selective pressures.

关 键 词：牛心柿 SSR 遗传多样性 聚类分析 

分 类 号：S665.2[农业科学—果树学]