葡聚糖-聚乳酸接枝共聚物的合成及其药物载体输送系统  

Synthesis of Amphiphilic Dextran-Poly( Lactide) Copolymer and Their Nanoparticles as Doxorubicin Delivery Systems

作　　者：孙继红[1] 温丽利[2] 吴雁[3] 王欢[1] 李彦威[2] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100124 [2]太原理工大学化学化工学院,太原030024 [3]中国科学院国家纳米科学中心,北京100190

出　　处：《北京工业大学学报》2013年第8期1269-1274,共6页Journal of Beijing University of Technology

基　　金：国家重点基础研究发展计划项目(2009CB930200)

摘　　要：合成葡聚糖-聚乳酸接枝共聚物,并以此为载体,选择盐酸阿霉素为模型药物,利用纳米沉淀法和双乳法构建出了载药纳米粒子,并通过透射电镜(scanning electron microscope,TEM)、动态光散射(dynamic light scattering,DLS)、紫外(UV-vis)对其形貌、粒径及包封率进行了表征,结果表明:不同共聚物/药物比例会影响载药纳米粒子的粒径、粒径分布及包封率.此外,载药纳米粒子的体外释放实验表明:在弱碱条件(pH=7.4)下的释放速度比在弱酸条件(pH=5.0)下的慢,说明释放介质的pH对其释放性能有较大影响,有利于药物在肿瘤细胞中的控释.Novel biodegradable amphiphilic copolymer nanoparticles based on dextran and poly（lactide） have been prepared. To estimate the feasibility as drug carriers, an anti-tumor model drug doxorubicin （DOX） was successfully incorporated into polymeric nanoparticles by double emulsion （DE） and nanoprecipitation （NP） methods. The DOX-loaded nanoparticles were confirmed by dynamic light scattering （ DLS ）, transmission electron microscopy （ TEM ） and ultraviolet （ UV ） absorption spectrometry. The DOX-loaded nanoparticle size, size distribution and encapsulation efficiency （EE） were influenced by the feed weight ratio of the copolymer to DOX. In addition, in vitro release experiments exhibited the release behavior was affected by pH of release media. The DOX-loaded nanoparticles release slower in pH 7.4 than in pH 5.0 buffer. The dextran-co-PLA polymeric nanoparticles can be useful as drug carriers for anti-tumor drug delivery.

关 键 词：葡聚糖 丙交酯 接枝共聚物 纳米粒子 体外释放 

分 类 号：O629.12[理学—有机化学]