增液承气汤对津亏便秘衰老模型小鼠皮肤超微结构及透明质酸含量的影响  

Effects of Zengyechengqitang on skin morphology and the content of hyaluronic acid in the constipated aged mice model with syndrome of fluid-deficiency

作　　者：彭圆[1] 张翀[1] 张丽君[1] 田代志[2] 郭岚[2] 王平[2] 

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430065 [2]湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,湖北武汉430065

出　　处：《时珍国医国药》2013年第7期1573-1575,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2009CB522707)

摘　　要：目的观察增液承气汤对津亏便秘衰老模型小鼠皮肤超微结构及透明质酸含量的影响,为肺与大肠相表里的归经药物研究提供支持。方法将大鼠随机分为空白对照组、模型组、西沙必利组、增液承气汤组、增液汤组。采用限水(1.5ml/d),叠加复方地芬诺酯[10 mg/(kg.d)]灌胃,同时10%D-半乳糖皮下注射[1 000 mg/(kg.d)]6周建立老年便秘模型。从第4周起分别给予相应药物灌胃干预3周。6周后取皮肤组织制备标本,光电镜观察皮肤组织的超微结构用放免法测皮肤透明质酸含量。结果与空白组比较,便秘衰老组皮肤透明质酸含量显著降低;皮肤明显变薄,胶原纤维数量减少;皮肤成纤维细胞存在明显线粒体水肿,异染色质发生边集,常染色质淡染或核染色致密成团的超微结构改变。与便秘衰老模型相比,增液承气汤组小鼠皮肤透明质酸含量明显增加;皮肤显著增厚,真皮胶原纤维数量增加,皮下脂肪填充减少;成纤维细胞损伤明显减轻,其核内染色质分布较均匀,核膜下异染色质聚集,胞浆内可见大量粗面内质网及线粒体。结论增液承气汤可有效改善模型小鼠皮肤组织改变,增加透明质酸含量,具有延缓皮肤老化作用。Objective It is to observe the effects of Zengyechengqitang on skin morphology and the content of hyaluronic acid（HA） in the constipated aged mice model with syndrome of fluid-deficiency.Methods The mice of blank group are given ultrapure water every day,while four groups are given 1.5 ml intragastric administration in sequence as follows： the ultra-pure water [20 ml/（kg.d） ],diphenoxylate[10 ml/（kg.d） ],ultra-pure water（to add the remaining amount）,an interval of 3 hours every day,simultaneously with 0.1ml /10g 1000mg / kg D-galactose in the napes and backs continuously for 42 days to establish the subacute aged constipation models.Since Zengyechengqitang（0.6 g / ml）,Zengyetang（0.51g / ml） and Cisapride group（2.5mg / kg） have been established model for three weeks,we give corresponding gavage therapy with 0.2ml /10g for three weeks.After 6 weeks,optical and transmission electron microscopic（TEM） examination of the skin tissue was also performed to observe ultrastructures and the content of HA in skin is investigated with radio-immunity method.Results Compared with the blank group,the aged constipation group show the HA content of skin tissue decrease sharply（P 0.01）,and that skin become thinner,the amount of collagenous fibers in dermal significantly decrease.There is obvious mitochondrial swelling,chromatin margination occur.Pale staining euchromatin or nuclear staining of the ultrastructural changes of dense clumps on skin fibroblasts by TEM.Compared with the aged constipation group,Zengyechengqitang group shows the HA content of skin tissue increase sharply（P 0.01）,and that skin becomes thicker,the amount of collagenous fibers in dermal significantly increases,and fat cells in subcutaneous is less.The damage to the cell is lighter than the model group by TEM,which chromatin in nucleus is well-distributed and heterochromatin is aggregated under the nuclear membrane and nucleoli is prominent.A large number of rough endoplasmic reticulum and mitochondria shows within the

关 键 词：增液承气汤 皮肤 透明质酸 形态学 

分 类 号：R285.5[医药卫生—中药学]