正交设计优化川续断中川续断皂苷Ⅵ的提取工艺  

Optimization of Extraction of Asperosaponin Ⅵ from Dipsacus asperoids by Orthogonal Experiment

作　　者：谷丹丹[1,2] 邓洪[1,2] 覃瑶[3] 李星颖[2] 施汀兰[2] 王欣[2] 罗维早[2] 

机构地区：[1]成都中医药大学,四川成都611730 [2]重庆市中药研究院,重庆400065 [3]太极集团有限公司,重庆401147

出　　处：《时珍国医国药》2013年第7期1587-1588,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技重大专项课题重庆创新药物孵化基地项目子课题(No.2010ZX09401-306-3-8);重庆市自然科学基金计划重点项目课题(No.CSTC;2009BA5079)

摘　　要：目的优选川续断中川续断皂苷Ⅵ的提取工艺。方法以川续断皂苷Ⅵ转移率为指标,采用单因素、正交试验的方法,优化提取工艺参数。结果选用回流法提取,6倍量65%乙醇连续回流提取3次,每次2h的提取效果最佳。结论乙醇回流提取简单且效率高,适用于川续断中川续断皂苷Ⅵ的提取。Objective To optimize the extractive process of asperosaponin Ⅵ in Dipsacus asperoids.Methods With the help of extraction rate of Asperosaponin Ⅵ,the optimal conditions were optimized by adopting a single factor as well as orthogonal experiment.Results The optimum extraction process was as follows： 6 times of 65% alcohol,refluxing 3 times,2 hours every time.Conclusion The reflux method was suitable for extraction of asperosaponin Ⅵ for its simple and high effectiveness.

关 键 词：川续断 川续断皂苷Ⅵ 回流法 

分 类 号：R284.2[医药卫生—中药学]