多杀性巴氏杆菌分离鉴定及序列分析  

Isolation,Identification and Sequence Analysis of Pasteurella multocida

作　　者：康立超[1] 黄新[1] 韩猛立[1] 王正荣[1] 何延华[1] 谭鹏飞[1] 薄新文[1] 钟发刚[1] 王新华[1] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,绵羊繁育生物技术重点实验室,新疆石河子832000

出　　处：《西北农业学报》2013年第6期10-16,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划(2012BAD43B00);新疆农垦科学院科技引导计划(YYD201107);新疆兵团绵羊繁育生物技术重点实验室开放课题(2011KLS04)

摘　　要：旨在了解新疆地区多杀性巴氏杆菌主要流行株的特性。通过病原的分离纯化、PCR扩增及测序,分析8株多杀性巴氏杆菌的血清型,16SrDNA基因、ompH基因和ompA基因的差异。结果表明,3株羊源、2株牛源和1株禽源分离株为荚膜A型,1株牦牛源分离株为荚膜B型,1株标准株为荚膜E型,均具有很强的致病性。分离株与GenBank公布的代表株的16SrDNA基因、ompH及ompA基因同源性分别为93%～100%、93%～100%、98%～100%。ompH基因的ORF氨基酸C端最后10个氨基酸变化较大,分离到5株ompA基因的ORF氨基酸序列N端多出6个氨基酸(M-K-R-I-I-Q)替代原先的起始位置。可见:新疆主要流行株为强致病性的荚膜A型,且有出现变异趋势。In order to understand the characteristics of the major epidemic strain of Pasteurella multocida in Xinjiang,the differences among serotype,16S rDNA,ompH and ompA gene sequence from eight Pasteurella multocida were examined and analyzed with isolation and PCR methods.The result showed that isolates from three sheep sources,two cattle sources and an avian source were capsular type A.A yak source was capsular type B.A standard strain was capsular type E.All of them were strong pathogenicity.The homology of 16S rDNA,ompH and ompA from eight Pasteurella multocida were 93%-100%,93%-100% and 98%-100% respectively.The last 10 amino acids in C-terminal of ompH gene ORF were different.The N-terminal was added six amino acids（M-K-R-I-I-Q）,which replaced the original starting sequences in ORF of ompA gene from the recently five isolates.The epidemic strains were strong pathogenic and capsular type A in Xinjiang.Based on these data,we speculate that the mutants would appear.

关 键 词：多杀性巴氏杆菌 16S RDNA ompH基因 ompA基因 序列分析 

分 类 号：S852.61[农业科学—基础兽医学]