清宫汤对小鼠注射氟马西尼后高架十字迷宫实验的影响  

The Effect of Qinggong Decoction on Mice injected flumazenil in the Elevated Plus-maze

作　　者：李越兰[1] 张世亮[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中国中医急症》2013年第7期1111-1112,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省教育厅项目(Y201016975)

摘　　要：目的观察清宫汤对小鼠注射氟马西尼后高架十字迷宫实验的影响,进一步探讨其抗焦虑作用机制。方法将小鼠随机分为正常对照组(生理盐水)、地西泮对照组(0.0015 g/kg)、清宫汤高、中、低剂量组(12、6、3 g/kg),灌胃给药,每日1次,连续10 d。测试前0.5 h,除空白组外,均腹腔注射氟马西尼(3 mg/kg),采用高架十字迷宫焦虑动物模型测试,观察清宫汤对小鼠注射氟马西尼后高架十字迷宫实验的影响。结果与空白对照组比较,地西泮组及清宫汤各组对注射氟马西尼后小鼠进入开放臂次数比(OE%)和在开放臂停留时间比(OT%)影响差异无统计学意义(P>0.05),地西泮及清宫汤的抗焦虑作用被氟马西尼所拮抗。结论清宫汤抗焦虑作用机制可能与GABA受体相关。Objective：To observe the effect of Qinggong Decoction（QGD） on mice injected flumazenil in elevated plus-maze,and to explore the anti-anxiety mechanisms.Methods：Mice were randomly divided into 5 groups：normal group（normal saline）,diazepam group（0.0015 g.kg-1）,QGD high-dose group（12 g.kg-1）,medium-dose group（6 g.kg-1） and low-dose group（3 g.kg-1）,and gavage administration once per day for10 days.Before the test 0.5 hour,all were intraperitoneal injected with flumazenil（3 mg/kg） except normal group,then the influence of QGD on the mice injected flumazenil were observed by adopting elevated plus-maze test.Results：Compared with normal group,the percentage of open arm entries（OE%） and percentage of time spent in the open arms（OT%） of diazepam group and QGD soups were no significant difference（P 0.05）,anti-anxiety of diazepam and QGT were subdued by flumazenil.Conclusion：The anti-anxiety mechanism of QGD may be related with GABA receptor.

关 键 词：清宫汤 高架十字迷宫 氟马西尼 抗焦虑 

分 类 号：R285.5[医药卫生—中药学]