替米沙坦对胰岛素抵抗大鼠心肌细胞脂联素受体的作用  

Effect of telmisartan on the expression of myocardial adiponectin receptor in insulin resistant rat

作　　者：郭春艳[1] 李虹伟[1] 沈潞华[1] 赵树梅[1] 

机构地区：[1]首都医科大学附属北京友谊医院心血管中心,北京100050

出　　处：《临床和实验医学杂志》2013年第14期1081-1083,共3页Journal of Clinical and Experimental Medicine

基　　金：北京市自然科学基金资助(基金项目编号:7082027)

摘　　要：目的观察替米沙坦对心肌细胞脂联素(APN)受体的影响,从而探讨胰岛素抵抗中心肌脂联素受体表达可能的调控机制。方法应用高果糖饮食造模成功的胰岛素抵抗(IR)大鼠,采用酶解法分离出心室肌细胞,进行原代细胞培养,将心室肌细胞分为两组:对照组予二甲基亚砜(DMSO)刺激24 h,替米沙坦组予DMSO+替米沙坦10μM/L刺激24 h。培养心肌细胞24 h后采用酶联免疫吸附法(ELISA)测定两组心室肌细胞培养液中APN的生成。采用Western Blot法测定不同处理后心肌细胞脂联素受体1(AdipoR1)、受体2(AdipoR2)、过氧化物酶体增殖物激活受体γ(PPARγ)、肿瘤坏死因子α(TNF-α)蛋白的表达。结果与对照组相比,替米沙坦组心室肌细胞培养液中脂联素的浓度增高(P<0.05)。与对照组相比,替米沙坦组心肌细胞AdipoR1蛋白的表达增高(P<0.05),而心肌细胞AdipoR2蛋白的表达无明显变化(P>0.05);心肌细胞PPARγ蛋白的表达增高(P<0.05),心肌细胞TNF-α蛋白的表达降低(P<0.05)。结论替米沙坦可以通过激活PPARγ提高胰岛素抵抗大鼠心肌细胞脂联素受体表达,减轻炎症,改善心肌代谢。脂联素及AdipoR1可能是其发挥作用的目标蛋白之一。Objective To explore the influence of telmisartan on the expression of myocardial adiponectin receptors.The second aim of this study was to elucidate the possible mechanism of angiotensin receptor blockers to ameliorate insulin resistance.Methods Male Sprague Dawley rats were fed with high fructose diet to induce IR rats.Then the isoated cardiomyocytes by enzymic method were treated with DMSO（control group） or telmisartan（10 μM / L） and DMSO（telmisartan group） for 24 hours.The APN concentration in the culture solution was detected by ELISA in both groups.The protein expression of myocardial AdipoR1,AdipoR2,PPARγ,TNF-α was detected by Western Blot in both groups.Results Compared with the control group,the APN concentration in the culture solution of telmisartan group was increased（P 0.05）.Compared with the control group,the AdipoR1 protein expression of cardiomyocytes in telmisartan group was increased（P 0.05）;whereas there was no difference in myocardial AdipoR2 protein expression（P 0.05）.The PPARγ protein expression was increased（P 0.05） The TNF-α protein expression was decreased（P 0.05）.Conclusion Telmisartan can increase the myocardial expression of AdipoR1,reduce inflammation and improve myocardial metabolism through activating PPARγ.APN and AdipoR1 may be one of its target proteins in cardiac tissue.

关 键 词：大鼠 胰岛素抵抗 脂联素 受体 心肌细胞 替米沙坦 

分 类 号：R587.1[医药卫生—内分泌]