鸡胚中禽波氏杆菌的分离鉴定及耐药性分析  

Isolation and Identification and Antibiotic Resistance of one Bordetella avium Isolate from Chick Embryos

作　　者：杨萍萍[1] 谭燕玲[1] 赵雪[1] 刘静静[1] 贺晓华[1] 刘冠华[1] 朱瑞良[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《河北科技师范学院学报》2013年第2期61-65,共5页Journal of Hebei Normal University of Science & Technology

基　　金：国家自然科学基金项目(项目编号:31272595;30972183)

摘　　要：从出现孵化率降低(80.3%)和弱雏率增多(16.5%)等现象的山东某大型海蓝褐种禽孵化场18日龄将出壳鸡胚中分离到1株禽波氏杆菌,命名为LL09株,并进行了23S rRNA的序列扩增和耐药性分析。结果表明,其23S rRNA序列与同年分离到的XT09株的同源性为100%,与其它8株B.avium的同源性为96.2%～96.6%,而与GenBank中收录的B.avium NC-010645的同源性为95.8%。该菌对所检测的14类47种药物中有28种呈现完全耐药,6种为低敏,只有13种药物比较敏感。耐受药物中包括了头孢类、青霉素类、单酰胺环类、大环内酯类、糖肽类、磺胺类等常用药物,只有喹诺酮类、氨基糖苷类和呋喃类中的部分药物的杀菌效果较好。Decrease of Hatchability （ 80. 3% ） and increase of weak chicken rate （ 16.5% ） emerged in a large Highland Brown breeding poultry hatchery of Shandong province, and one Bordetella avium strain was i- solated from its 18-day-old chick embryos and was named isolate LL09. Then its 23S rRNA sequence was am- plified and antibiotic resistance was analyzed. The results showed that the 23S rRNA sequence from isolate LL09 showed maximum similarity of 100% with the gene from strain XT09, 96.2% - 96.6% with the other eight B. avium strains, and 95.8% with NC-010645. Among 47 detected drugs, this isolate showed complete- ly resistant to 28 drugs, low-sensitive to 6 drugs and only sensitive to 13 drugs. Resistant drugs included ceph- alosporins, penieinins, Monocyclic lactam, macrolides, glyeopeptides, sulfa etc. Only effects of part of quin- olones, aminoglycosides and furans were perfect.

关 键 词：禽波氏杆菌 鸡胚 分离鉴定 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]