诱导型一氧化氮合酶基因多态性与慢性中重度牙周炎的相关性分析  

Correlation between polymorphism of inducible nitric oxide synthase gene and chronic severe periodontitis

作　　者：丁媛媛[1] 边佳明[1] 张秋丽[1] 赵文峰[1] 许景峰[1] 杨素芳 

机构地区：[1]北京军区总医院,100700 [2]北京总政机关门诊部

出　　处：《山西医药杂志（下半月）》2013年第7期734-736,共3页Shanxi Medical Journal

基　　金：军队十二五课题资助项目(CWS11G038)

摘　　要：目的研究诱导型一氧化氮合酶(iNOS)基因外显子Ser608Leu位点多态性与慢性中重度牙周炎的相关性。方法收集中重度慢性牙周炎患者116例和健康体检对照组80名,测序法比较2组间iNOS基因Ser608Leu位点基因型及等位基因频率,同时用酶联免疫吸附试验(ELASA)法测定血清iNOS活性,评估iNOS基因多态性与中、重度慢性牙周炎炎症反应的相关性。结果 iNOS基因Ser608Leu位点AA型在中重度牙周炎和对照组的分布差异具有统计学意义(P<0.05)。A和G等位基因在中重度牙周炎和对照组的分布差异具有统计学意义(P<0.05),具有AA型的牙周炎患者患病的风险是其他基因型的1.45倍(P<0.05)。AA型患者血清iNOS水平与AG和GG型相比差异具有统计学意义(P<0.05)。结论 iNOS基因Ser608Leu位点多态性与中重度慢性牙周炎易感性和炎症反应有关。Objective To observe the relationship between inducible nitric oxide synthase （iNOS） gene Ser608Leu allele polymorphism and chronic periodontitis.Methods A total of 116 chronic periodontitis patients were enrolled,and 80 healthy people from examination subjects were as a control group. To evaluate correlation between polymorphism of iNOS gene Ser608Leu and chronic severe periodontitis, DNA sequencing method was used to detect G to A mutation of gene and ELASA method was used to detect the active of iNOS in serum. iNOS gene Ser608Leu polymorphisms of the two groups were compared.Results The distribution of AA genotype between periodontitis and the control group has significant difference （P0.05）. A and G allele distribution between chronic severe periodontitis and the control group has significant difference （P0.05）, The risk of AA genotype with periodontitis was 1.45 times than that of other genotypes （P0.05）. There were statistical differences in the level of serum iNOS between AA genotype and AG or GG genotype in chronic severe periodontitis group（P0.05）. Conclusion Our study reveals some possible influence of single-nucleotide polymorphisms for iNOS Ser608Leu in chronic severe periodontitis.

关 键 词：牙周炎 多态性 单核苷酸 一氧化氮合酶 

分 类 号：R743.31[医药卫生—神经病学与精神病学]