NMDA受体拮抗剂MK-801对新生大鼠室管膜下区胶质纤维酸性蛋白表达的影响  

Effects of N-methyl-D-aspartateReceptorAntagonistMK-801onExpressionofGlialFibrillaryAcidic Protein in the Subventricular Zone of Neonatal Rat

作　　者：李晓泉[1] 陈芳芳[1] 王伟[1] 来青伟[1] 耿德勤[1] 樊红彬[1] 

机构地区：[1]徐州医学院附属医院神经内科,210002

出　　处：《中国临床神经科学》2013年第4期389-393,共5页Chinese Journal of Clinical Neurosciences

摘　　要：目的研究N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801对新生7 d大鼠室管膜下区(SVZ)胶质纤维酸性蛋白(GFAP)表达的影响。方法将40只新生SD大鼠分成对照组和MK-801组,各组按出生后(P)时间点再随机分成4个亚组:P7 d、P14 d、P21 d、P28 d组。新生大鼠均于生后第3天给药,MK-801组腹腔注射MK-801 10 mg.kg-1;对照组腹腔注射同量生理盐水。通过免疫组化学方法观察大鼠SVZ区GFAP阳性细胞数。结果①对照组GFAP阳性细胞数于P14 d开始增加,至P21 d达最大值;但P28 d时阳性细胞迅速下降;②MK-801组GFAP阳性细胞数与对照组相比,P7 d和P28 d无明显差异,P14 d(65.40±6.11)和P21 d(239.60±12.92)细胞数明显减少;而对照组P14 d(79.20±5.26)、P21 d(265.20±7.40)GFAP阳性细胞数明显增多,差异有显著统计学意义(P<0.01)。结论 NMDA受体拮抗剂MK-801对正常新生大鼠SVZ区GFAP的表达有抑制作用,能够抑制SVZ区神经干细胞的增殖和分化。Aim To investigate the effects of the N-methyl-D-aspartate（NMDA）-receptor antagonist MK-801 on the expression of glial fibrillary acidic protein（GFAP） in the subventricular zone（SVZ） of neonatal rat. Methods 40 neonatal SD rats were assigned into two groups, a MK-801 group and a control group. Each group was respectively divided into four time points, including P7 d （postnatal 7 days）, P14 d, P21 d and P28 d. Rats in the experiment group were intraperitoneally injected selective non-competitive NMDA receptor antagonist MK-801 （10 mg·g-1）, while rats in the control group were intraperitoneally injected saline. Immunohistochemical staining was used to assay the number of GFAP-positive cells in the SVZ. Results ①In the control group, the number of GFAP-positive cells increased at P14 d, and the number of GFAP-positive cells reached maximum at P21 d, positive cells decreased significantly at 28 d. ②Compared with the control group, the number of GFAP-positive cells reduced significantly at P14 d （65.40±6.11） and P21 d （239.60±12.92）, and the number of GFAP-positive cells increased significantly [P14 d（79.20±5.26）, P21 d（265.20±7.40）]（P0.01）, while the number of positive cells without obvious changes at P7 d and P28 d in the MK-801 group. Conclusion NMDA-receptor antagonist MK-801 could reduce the expression of GFAP in the SVZ, and inhibit the neural stem cells proliferation and differentiation.

关 键 词：胶质纤维酸性蛋白 室管膜下区 N-甲基-D-天冬氨酸受体拮抗剂 神经干细胞 细胞增殖 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] Q189[医药卫生—基础医学]