GATA-4过表达对大鼠骨髓间充质干细胞保护心肌细胞的影响  

Effects of GATA-4 overexpression on cardiomyocytes protected by bone marrow mesenchymal stem cells in rats

作　　者：李红霞[1] 周亚峰[1] 蒋彬[1] 杨向军[1] 蒋文平[1] 

机构地区：[1]苏州大学附属第一医院心脏病科,江苏省215006

出　　处：《江苏医药》2013年第15期1737-1739,F0003,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金资助(81100173;81070139);江苏省普通高校研究生科研创新计划资助(CXZZ11_0118);江苏省医学创新团队与领军人才项目(LJ201140)

摘　　要：目的研究GATA-4转染骨髓间充质干细胞(BMSCs)对心肌细胞(CM)保护的影响。方法体外分离培养BMSCs及CM。实验分为常氧培养CM(A)组、低氧培养CM(B)组、低氧+CM+BMSCsNull(C)组和低氧+CM+BMSCsGATA-4(D)组。倒置显微镜下观察CM形态变化,试剂盒检测乳酸脱氢酶(LDH)释放、MTT摄取及膜联蛋白V阳性细胞数。结果与A组相比,B组大部分CM明显收缩变圆,LDH释放增多,MTT摄取减少,膜联蛋白V阳性细胞数增加(P<0.05);而C组和D组能改善B组上述各指标变化过程,其中D组作用更为明显(P<0.05)。结论 GATA-4过表达BMSCs与CM共培养可以通过降低LDH释放、增加MTT摄取和降低膜联蛋白V阳性细胞数来保护CM。Objective To investigate the effects of GATA-4transfection on cardiomyocytes（CM）protected by mesenchymal stem cells（BMSCs）in rats.Methods BMSCs and CMs were isolated and cultured in vitro,and then divided into four groups of A（CM exposed to normoxia）,B（CM exposed to hypoxia）,C（co-culture of CM and BMSCsNull exposed to hypoxia）and D（co-culture of CM and BMSCsGATA-4 exposed to hypoxia）.The morphologic change of CM was observed under inverted microscope.The cell injury markers such as LDH release,MTT uptake and positive cells of annexin-V were detected by kits.Results Compared with group A,the majority of CM were shrank and became round,LDH release and positive cells of annexin-V were increased,while MTT uptake was decreased in group B（P〈0.05）,which were all improved in groups of C and D,especially in group D（P〈0.05）.Conclusion GATA-4overexpression in co-culture of CM and BMSCs can protect CM via increasing MTT uptake and decreasing LDH release and positive cells of annexin-V.

关 键 词：骨髓间充质干细胞 心肌细胞 GATA-4 心肌保护 

分 类 号：R541[医药卫生—心血管疾病]