十二指肠空肠旁路术对非肥胖2型糖尿病大鼠小肠糖异生相关基因表达的影响  

Effect of duodenal-jejunal bypass surgery on the expression of intestinal gluconeogenesis genes in nonobese type 2 diabetic rats

作　　者：张鹏翎[1,2] 马向华 桑谊荃 谢媛 田婷 张媛媛 管蔚 梁辉 沈捷 

机构地区：[1]南京医科大学第一附属医院内分泌病科,江苏省210029 [2]江苏省省级机关医院内分泌病科 [3]南京医科大学第一附属医院普通外科,江苏省210029 [4]南京医科大学第一附属医院HLA实验室,江苏省210029

出　　处：《江苏医药》2013年第15期1742-1744,I0001,共4页Jiangsu Medical Journal

摘　　要：目的探讨十二指肠空肠旁路术对非肥胖2型糖尿病GK大鼠小肠糖异生相关基因葡萄糖-6-磷酸酶(G-6-P)、磷酸烯醇丙酮酸羧激酶(PEPCK)mRNA表达的影响。方法非肥胖2型糖尿病GK大鼠及正常Wistar大鼠各16只,随机分为GK大鼠手术组(A组)、GK大鼠假手术组(B组)、Wistar大鼠手术组(C组)及Wistar大鼠假手术组(D组),每组各8只。术后第8周取大鼠十二指肠段的小肠组织,RT-PCR检测G-6-P和PEPCK mRNA表达量。结果A组PEPCK和G-6-PmRNA表达量显著高于B组(P<0.01)。C组与D组G-6-P和PEPCK mRNA表达量比较差异无统计学意义。结论十二指肠空肠旁路术能够上调GK大鼠小肠糖异生相关基因G-6-P和PEPCK基因的表达,促进小肠糖异生的发生。Objective To investigate the effect of duodenal-jejunal bypass（DJB）on the mRNA expressions of intestine glucose-6-phosphatase（G-6-P）and phosphoenolpyruvatecarboxylase（PEPCK） in non-obese type 2diabetic rats.Methods A total of 16GK rats and 16 Wistar rats was randomly divided into GK operation（group A）,GK sham operation（group B）,Wistar operation（group C）and Wistar sham operation（group D）with 8rats each.At the end of 8 weeks after operation,the duodenum of small intestine was taken out for the detections of G-6-P and PEPCK genes mRNA expressions by RT-PCR.Results Compared to group B,PEPCK mRNA expression was significantly increased in group A（P〈0.01）.No statistically significant differences of PEPCK and G-6-P mRNA expression were shown between groups of C and D.Conclusion DJB can upregulate G-6-P and PEPCK genes expression of small intestine in GK rats and promote small intestinal gluconeogenesis.

关 键 词：小肠糖异生 葡萄糖-6-磷酸酶 磷酸烯醇丙酮酸羧基酶 

分 类 号：R587[医药卫生—内分泌]