设计及自组装六边形网格状DNA二维阵列  

Design and self-assembly of hexagonal latticed DNA two-dimensional arrays

作　　者：庄立洲[1] 周明[1,2] 杨晓红[1] 叶日安[1] AMOAKO George 沈智勇[1] 

机构地区：[1]江苏大学光子制造科学技术中心,镇江212013 [2]清华大学摩擦学国家重点实验室,北京100084

出　　处：《科学通报》2013年第21期2094-2099,共6页Chinese Science Bulletin

基　　金：国家重点基础研究发展计划(2011CB013004);清华大学摩擦学国家重点实验室自主研究重点项目(SKLT10A02)资助

摘　　要：根据Watson-Crick DNA碱基互补配对原则,设计了不同黏性末端DNA序列,以两种三点星状为模块(three-point-star motif),运用DNA自组装技术,成功制备了六边形网格状DNA二维阵列.研究不同单链DNA的摩尔比例对三点星状模块结构组装的影响,以及不同起始退火温度对二维阵列自组装的影响,用凝胶电泳及原子力显微镜(AFM)对结构进行了表征.结果表明,当构成三点星状模块的3条单链摩尔比例为1:3:3时得到的模块结构更稳定,从50℃开始退火时得到的二维阵列更完整.自组装得到的二维阵列厚度约2nm,六边形边长约20nm.本研究为进一步探究三点星模块组装二维阵列的机理提供了新的思路.Hexagonal lattices of two-dimensional （2D） DNA arrays were prepared via two kinds of three-point-star motifs on the basis of Watson- Crick complementarity. We studied the influence of different molar ratio of single-stranded DNA on the structure of three-point-star motif and the influence of different annealing temperature on the self-assembly of 2D arrays with gel electrophoresis and atomic force microscopy （AFM）. The results showed that the structure of three-point-star motifs is more stable when the molar ratio of three single-stranded DNA is 1 ： 3 ： 3, corresponding to the stoichiometry of the assembly ratio. The 2D arrays are integrated better when the annealing temperature is 50~C. The height of the array is 2 nm; and the length of hexagonal side is 20 nm. The mechanism of three-point-star motif self-assembly to 2D arrays was suggested.

关 键 词：DNA三点星状 模块 二维阵列 自组装 凝胶电泳 原子力显微镜 

分 类 号：Q523[生物学—生物化学]