人白细胞抗原基因多态性与乙型肝炎疫苗远期免疫应答水平的关系  

HLA gene polymorphisms and long-term humoral response in children immunized with hepatitis B vaccine at birth

作　　者：朱冬梅[1,2] 江剑华[1] 毕永春[2] 张姝[2] 胡娅莉[2] 周乙华[2] 

机构地区：[1]江苏省常州市第三人民医院,江苏常州213001 [2]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《中国病毒病杂志》2013年第4期293-296,共4页Chinese Journal of Viral Diseases

基　　金：南京市第一层次卫生青年人才培养项目(2011024)

摘　　要：目的探讨人白细胞抗原(HLA)基因多态性与新生儿接种乙型肝炎(乙肝)疫苗远期体液免疫应答水平的相关性。方法以新生儿期按0、1、6月方案接种过乙肝疫苗的297名儿童为研究对象,其中86名抗-HBs<10mIU/ml的儿童为远期无/弱应答组,211名抗-HBs≥10mIU/ml的儿童为远期应答组。采用序列特异性引物聚合酶链反应(PCR-SSP)技术,对HLA-DR和DQ亚区共10个等位基因进行分型。结果 HLA-DRB1*04和DRB1*07在远期无/弱应答组的频率为27.9%和36.0%,分别高于远期应答组的18.5%和27.0%,但差异无统计学意义(P值分别为0.072和0.122);HLA-DQB1*0401在远期无/弱应答组的频率为15.1%,高于远期应答组的7.6%(P=0.047)。其余DR亚区的5个等位基因和DQ亚区的2个等位基因在远期无/弱应答组和远期应答组的分布相近。结论 HLA-DQB1*0401可能与儿童在新生儿期接种乙肝疫苗后远期低水平抗体应答相关。Objective To study the association between human leukocyte antigen （HLA） class II gene poly- morphisms and the long-term humoral response in children vaccinated with hepatitis B vaccine at birth. Methods A total of 297 children between 5 to 7 years old who were immunized with hepatitis B vaccine at birth with the 0, 1-, and 6-month schedule were enrolled. Of them, 86 children with anti-HBs〈10 mIU/ml were con- sidered as long term non and hypo-responders; the remaining 211 subjects with anti-HBs≥10 mIU/ml were defined as long-term responders. A total of 10 alleles in HLA-DR and-DQ subregions were detected with poly- merase chain reaction （PCR） using sequence-specific primers. Results The frequencies of HLA-DRB1＊ 04 （27.9%） and DRB1 ＊ 07 （36.0%） in long term non and hypo-responders were higher than that in responders （18.5% and 27.0%, respectively） but with no statistical significance （P=0. 072 and 0. 122, respectively）. The frequency of HLA-DQB1 ＊ 0401 in long-term non-and hypo-responders was significantly higher than that in responders （15.1 % vs 7.6%, P=0. 047）. The allele distributions of five other alleles in the DR subregion and two other alleles in the DQ subregion between long-term non-and hypo-responders and responders were similar. Conclusions HLA-DQB1＊ 0401 is associated with the long-term humoral response in children vacci- nated against hepatitis B during infancy.

关 键 词：人白细胞抗原 基因多态性 乙型肝炎疫苗 抗-HBs远期应答 

分 类 号：R512.62[医药卫生—内科学]