二甲磺酸乙烷对成年大鼠精囊组织形态结构的影响  

Impact of ethane dimethane sulfonate on the histological structures of seminal vesicles in adult rats

作　　者：郭洋[1] 杨正伟[1] 

机构地区：[1]川北医学院形态定量研究室,四川南充637007

出　　处：《中华男科学杂志》2013年第8期678-682,681-682,共5页National Journal of Andrology

基　　金：四川省青年科技基金会资助项目(04ZQ026-025)~~

摘　　要：目的:研究二甲磺酸乙烷(EDS)注射对大鼠精囊不同组织结构体积的影响。方法:27只约90日龄雄性SD大鼠随机分成2组,对照组(n=14)一次性腹腔内注射生理盐水,EDS组(n=13)一次性腹腔内注射EDS75 mg/kg体重;7 d和12 d后取一侧精囊,制作甲基丙烯酸树脂包埋切片,用体视学方法估计精囊内各种结构的总体积。结果:EDS几乎完全破坏睾丸内的Leydig细胞,导致急剧睾酮缺乏。与7 d对照组(n=7)相比,7 d EDS组(n=6)精囊(包括附着在精囊上的凝固腺)的体积减少53%[(138.2±12.9)mm3vs(64.9±3.6)mm3,P<0.05],但该体积在7 d EDS组和12 d EDS组(n=7)之间无显著差异[(64.9±3.6)mm3vs(55.4±7.7)mm3,P>0.05]。与12 d对照组(n=7)相比,12 d EDS组腺腔、腺上皮、平滑肌层和外膜的总体积分别显著减少97%、80%、58%和67%。结论:EDS处理导致大鼠精囊腺腔、腺上皮、平滑肌层和外膜结构的严重萎缩。Objective： To study the effect of ethane dimethane sulfonate （EDS） injection on the volumes of different histologi- cal structures in the seminal vesicles of adult rats. Methods： Twenty-seven male SD rats aged approximately 90 days were randomly divided into a control group （n = 14） and an EDS group （n = 13 ） to receive one intraperitoneal injection of normal saline and EDS （75 mg/kg bodyweight）, respectively. At 7 and 12 days after treatment, the unilateral seminal vesicles were removed, methacrylate resinembedded sections prepared and the total volumes of various structures in the seminal vesicles estimated using stereological methods. Results： EDS treatment almost completely destroyed the Leydig cells in the testis, resulting in a drastic testosterone deficiency. The volume of the seminal vesicle （ including the coagulating gland attached to the vesicle） was decreased by 53% in the 7 d EDS group （ n = 6） in comparison with the 7 d control group （ n = 7 ） （ [ 138.2 ± 12.9 ] vs [ 64.9 ± 3.6 ] mm3, P 〈 0.01 ）, but showed no significant difference between the 7 d and the 12 d EDS （ n = 7） groups （ [ 64.9 ± 3.6 ] vs [ 55.4 ± 7.7 ] mm3, P 〉 0.05 ）. The total volumes of the glandular lumen, glandular epithelium, smooth muscular layer and adventitia were decreased by 96.7, 80.3, 57.6 and 67.0%, respectively, in the 12 d EDS group as compared with the 12 d control group （n = 7）. Conclusion： EDS induces drastic testosterone deficiency in adult rats, and significantly reduces the total volumes of the seminal vesicle lumen, glandular epithelium, smooth muscular layer and adventitia.

关 键 词：二甲磺酸乙烷 精囊 组织学 体视学 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]